Lung microbicidal mechanisms and pulmonary hypertension in septic piglets
These studies sought to determine whether the pulmonary hemodynamic abnormalities elicited in infant piglets in response to an intravascular bacterial challenge are dependent upon the clearance and killing of the organism by an oxygen radical dependent mechanism residing within the pulmonary circulation of the host. Initial studies were conducted to ascertain the effect of dimethylthiourea (DMTU), a relatively selective scavenger of hydroxyl radical, on Group B Streptococcus (GBS)-induced pulmonary abnormalities in young piglets. Lung samples from piglets infused with GBS and evaluated by transmission electron microscopy revealed that the GBS had been taken up by pulmonary intravascular macrophages. To determine clearance characteristics of intravascularly administered GBS as well as oxygen radical involvement in pulmonary bactericidal mechanisms, studies were performed in which GBS was radiolabelled with {sup 111}Indium-oxine and infused into piglets in the presence or absence of DMTU. In accord with the previous study, animals receiving GBS only exhibited profound increases in Ppa and TPR with concomitant arterial hypoxemia, all of which were attenuated by the co-administration of DMTU. Bacterial accumulated to the greatest extent in the lung, followed by the liver and spleen.
- Research Organization:
- Kentucky Univ., Lexington, KY (USA)
- OSTI ID:
- 6617267
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HYDROXYL RADICALS
BIOLOGICAL EFFECTS
HYPERTENSION
PATHOGENESIS
STREPTOCOCCUS
LUNG CLEARANCE
BACTERIAL DISEASES
BLOOD PRESSURE
INDIUM 111
LUNGS
MACROPHAGES
OXINE
SWINE
THIOUREAS
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
AZINES
BACTERIA
BETA DECAY RADIOISOTOPES
BODY
CARBONIC ACID DERIVATIVES
CARDIOVASCULAR DISEASES
CLEARANCE
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
EXCRETION
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM ISOTOPES
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MICROORGANISMS
MICROSCOPY
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PHAGOCYTES
PYRIDINES
QUINOLINES
RADICALS
RADIOISOTOPES
RESPIRATORY SYSTEM
SOMATIC CELLS
SYMPTOMS
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques