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Title: Sympathetic neural modulation of the immune system

One route by which the central nervous system communicates with lymphoid organs in the periphery is through the sympathetic nervous system (SNS). To study SNS regulation of immune activity in vivo, selective removal of peripheral noradrenergic nerve fibers was achieved by administration of the neurotoxic drug, 6-hydroxydopamine (6-OHDA), to adult mice. To assess SNS influence on lymphocyte proliferation in vitro, uptake of {sup 125}iododeoxyuridine ({sup 125}IUdR), a DNA precursor, was measured following 6-OHDA treatment. Sympathectomy prior to epicutaneous immunization with TNCB did not alter draining lymph nodes (LN) cell proliferation, whereas 6-OHDA treatment before footpad immunization with KLH reduced DNA synthesis in popliteal LN by 50%. In mice which were not deliberately immunized, sympathectomy stimulated {sup 125}IUdR uptake inguinal and axillary LN, spleen, and bone marrow. In vitro, these LN and spleen cells exhibited decreased proliferation responses to the T cell mitogen, concanavalin A (Con A), whereas lipopolysaccharide (LPS)-stimulated IgG secretion was enhanced. Studies examining {sup 51}Cr-labeled lymphocyte trafficking to LN suggested that altered cell migration may play a part in sympathectomy-induced changes in LN cell function.
Authors:
Publication Date:
OSTI Identifier:
6617136
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
Rochester, NY (US); Univ. of Rochester
Research Org:
Rochester Univ., NY (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AUTONOMIC NERVOUS SYSTEM; BIOLOGICAL FUNCTIONS; AUTONOMIC NERVOUS SYSTEM AGENTS; BIOCHEMICAL REACTION KINETICS; LYMPHOCYTES; CELL PROLIFERATION; BONE MARROW; CONCANAVALIN; DEOXYURIDINE; DNA REPLICATION; IMMUNOGLOBULINS; IN VIVO; IODINE 125; LIPOPOLYSACCHARIDES; LYMPH NODES; MICE; RETICULOENDOTHELIAL SYSTEM; SECRETION; TRACER TECHNIQUES; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; ANTIMETABOLITES; AZINES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; FUNCTIONS; GLOBULINS; HEMATOPOIETIC SYSTEM; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LEUKOCYTES; LIPIDS; LYMPHATIC SYSTEM; MAMMALS; MATERIALS; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACID REPLICATION; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; POLYSACCHARIDES; PROTEINS; PYRIMIDINES; RADIOISOTOPES; REACTION KINETICS; RIBOSIDES; RODENTS; SACCHARIDES; SOMATIC CELLS; TISSUES; URACILS; VERTEBRATES 551001* -- Physiological Systems-- Tracer Techniques