Quantitative evaluation of radiation-induced changes in sperm morphology and chromatin distribution
- Institut fuer Strahlenschutz, Neuherberg (Germany, F.R.)
Sperm head cytometry provides a useful assay for the detection of radiation-induced damage in mouse germ cells. Exposure of the gonads to radiation is known to lead to an increase of diploid and higher polyploid sperm and of sperm with head shape abnormalities. In the pilot studies reported here quantitative analysis of the total DNA content, the morphology, and the chromatin distribution of mouse sperm was performed. The goal was to evaluate the discriminative power of features derived by high resolution image cytometry in distinguishing sperm of control and irradiated mice. Our results suggest that besides the induction of the above mentioned variations in DNA content and shape of sperm head, changes of the nonhomogeneous chromatin distribution within the sperm may also be used to quantify the radiation effect on sperm cells. Whereas the chromatin distribution features show larger variations for sperm 21 days after exposure (dpr), the shape parameters seem to be more important to discriminate sperm 35 dpr. This may be explained by differentiation processes, which take place in different stages during mouse spermatogenesis.
- OSTI ID:
- 6615688
- Journal Information:
- Cytometry (Baltimore); (USA), Vol. 11:5; ISSN 0196-4763
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMATIN
TISSUE DISTRIBUTION
SPERMATOZOA
BIOLOGICAL RADIATION EFFECTS
DNA
MICE
MORPHOLOGICAL CHANGES
RADIATION DOSES
SCANNING ELECTRON MICROSCOPY
SPERMATOGENESIS
ANIMALS
BIOLOGICAL EFFECTS
DISTRIBUTION
DOSES
ELECTRON MICROSCOPY
GAMETES
GAMETOGENESIS
GERM CELLS
MAMMALS
MICROSCOPY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals