Interlaboratory studies with the Chinese hamster V79 cell metabolic cooperation assay to detect tumor-promoting agents
Three laboratories participated in an interlaboratory study to evaluate the usefulness of the Chinese hamster V79 cell metabolic cooperation assay to predict the tumor-promoting activity of selected chemical. Twenty-three chemicals of different chemical structures (phorbol esters, barbiturates, phenols, artificial sweeteners, alkanes, and peroxides) were chosen for testing based on in vivo promotion activities, as reported in the literature. Assay protocols and materials were standardized, and the chemicals were coded to facilitate unbiased evaluation. A chemical was tested only once in each laboratory, with one of the three laboratories testing only 15 out of 23 chemicals. Dunnett's test was used for statistical analysis. Chemicals were scored as positive (at least two concentration levels statistically different than control), equivocal (only one concentration statistically different), or negative. For 15 chemicals tested in all three laboratories, there was complete agreement among the laboratories for nine chemicals. For the 23 chemicals tested in only two laboratories, there was agreement on 16 chemicals. With the exception of the peroxides and alkanes, the metabolic cooperation data were in general agreement with in vivo data. However, an overall evaluation of the V79 cell system for predicting in vivo promotion activity was difficult because of the organ specificity of certain chemicals and/or the limited number of adequately tested nonpromoting chemicals.
- Research Organization:
- National Institute for Occupational Safety and Health, Research Triangle Park, NC (USA)
- OSTI ID:
- 6615151
- Journal Information:
- Environ. Mol. Mutagen.; (United States), Vol. 12:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
TUMOR PROMOTERS
INTERLABORATORY COMPARISONS
ALKANES
BARBITURATES
FIBROBLASTS
HAMSTERS
IN VIVO
METABOLISM
PEROXIDES
PHENOLS
PHORBOL ESTERS
ANESTHETICS
ANIMAL CELLS
ANIMALS
AROMATICS
AZINES
CARCINOGENS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CONNECTIVE TISSUE CELLS
DRUGS
ESTERS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXY COMPOUNDS
HYPNOTICS AND SEDATIVES
MAMMALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXYGEN COMPOUNDS
PROMOTERS
PYRIMIDINES
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology