Identification of a ZP3-binding protein on acrosome-intact mouse sperm by photoaffinity crosslinking

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.14.5563· OSTI ID:6611522

Bleil, J D; Wassarman, P M ^[1]

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1. Roche Institute of Molecular Biology, Nutley, NJ (USA)

During the process of fertilization in mammals, sperm bind in a relatively species-specific manner to the zona pellucida (ZP) of ovulated eggs. ZP3, a glycoprotein found in the mouse egg zona pellucida, serves as receptor for sperm during gamete adhesion. We report here that a Mr 56,000 protein found on mouse sperm has properties expected for a sperm component that recognizes and binds to ZP3. This sperm protein is radiolabeled preferentially by a photoactivatable heterobifunctional crosslinker (Denny-Jaffee reagent) covalently linked to purified ZP3, binds very tightly to ZP3-affinity columns, and is localized to heads of acrosome-intact but not acrosome-reacted sperm. These and other findings suggest that this protein may be a ZP3-binding protein that, together with the sperm receptor, supports species-specific binding of mouse sperm to unfertilized eggs.

• OSTI ID: 6611522
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:14; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOPROTEINS
CHEMICAL PROPERTIES
SPERMATOZOA
ADHESION
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CROSS-LINKING
EGGS
ELECTROPHORESIS
IODINE ISOTOPES
MICE
REAGENTS
RECEPTORS
ANIMALS
CHEMICAL REACTIONS
GAMETES
GERM CELLS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
POLYMERIZATION
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques