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Title: Attempting to monitor the incorporation of deuterium into indole-3-acetic acid and tryptophan in Zea mays grown on deuterium oxide labeled water

We are attempting to determine when seedlings of Zea mays sweet corn, var. Silver Queen begin de novo biosynthesis of tryptophan and indole-3-acetic acid (IAA). We wish to use the general precursor, deuterium labeled water, to minimize assumptions as to the biosynthetic route. Protium in positions 2, 4, 5, 6 7 of the indole ring are non-exchangeable. IAA and tryptophan synthesized via the shikimic acid pathway would contain deuterium in one or more of these positions . The protium on the indene nitrogen, the carboxyl, the amino group, or the protium alpha to the carboxyl exchange readily and so are removed prior to analysis by base catalyzed exchange. The IAA, or trypotophan, is then purified by DEAE, Dowex 50, and two HPLC steps. IAA is methylated with diazomethane and analyzed by GC/MS. Trytophan is acetylated with triethylamine-acetic anhydride and then methylated with diazomethane and analyzed by GC/MS. Results of these studies on plants grown for varying lengths of time and under various light and nutrient conditions will be reported.
Authors:
;  [1]
  1. (Michigan State Univ., East Lansing (USA))
Publication Date:
OSTI Identifier:
6610935
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Physiology, Supplement; (USA); Journal Volume: 89:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AUXINS; BIOSYNTHESIS; TRYPTOPHAN; BIOLOGICAL PATHWAYS; DEUTERIUM; FRACTIONATION; GAS CHROMATOGRAPHY; HEAVY WATER; LIQUID COLUMN CHROMATOGRAPHY; MAIZE; MASS SPECTROSCOPY; TRACER TECHNIQUES; AMINO ACIDS; AROMATICS; AZAARENES; AZOLES; CARBOXYLIC ACIDS; CEREALS; CHROMATOGRAPHY; GRASS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; HYDROGEN ISOTOPES; INDOLES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LILIOPSIDA; MAGNOLIOPHYTA; NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXYGEN COMPOUNDS; PLANT GROWTH REGULATORS; PLANTS; PYRROLES; SEPARATION PROCESSES; SPECTROSCOPY; STABLE ISOTOPES; SYNTHESIS; WATER 550201* -- Biochemistry-- Tracer Techniques