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Title: Transformation by viral and cellular oncogenes of a mouse BALB/3T3 cell mutant resistant to transformation by chemical carcinogens

The mouse cell line MO-5 is resistant to transformation by various chemical carcinogens and also by UV irradiation. Northern (RNA) blot analysis showed active expression of ras and myc genes in MO-5 and BALB/3T3 cells. The effect of transfection of various oncogenes on transformation was compared in MO-5 cells and parental BALB/3T3 cells. Activated c-H-ras, c-N-ras, and v-mos gene induced transformation foci of MO-5 and BALB/3T3. Introduction of the polyomavirus middle T-antigen (mTag) or the Rous sarcoma virus-related oncogene v-src, however, efficiently transformed BALB/3T3 but not MO-5 cells. Expression and phosphorylation of mTag and the associated c-src proteins were observed in mTag-transfected clones of MO-5 as in BALB/3T3 and phosphorylation of the src protein was observed in v-src-transfected BALB/3T3 and MO-5 clones. Hybrids between mTag- or v-src-induced transformants of BALB/3T3 and untransformed MO-5 maintained the transformation phenotype, suggesting that no dominant suppressor of transformation exists in MO-5. A hybrid clone between BALB/3T3 and MO-5 induced efficient transformation foci after transfection with the mTag gene, suggesting that the deficient transformation phenotype of MO-5 was recessive. Instead, some other alteration of MO-5, plausibly membrane function, might lead to abortive transformation by chemical carcinogens and also by mTag and the v-src gene product.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6600483
Resource Type:
Journal Article
Resource Relation:
Journal Name: Mol. Cell. Biol.; (United States); Journal Volume: 8:10
Research Org:
Oita Medical School (Japan)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL CELLS; GENE REGULATION; ONCOGENES; BIOLOGICAL FUNCTIONS; PROTEINS; PHOSPHORYLATION; CARCINOGENS; HEXOSES; MICE; MUTANTS; ONCOGENIC TRANSFORMATIONS; PHENOTYPE; ANIMALS; CARBOHYDRATES; CELL TRANSFORMATIONS; CHEMICAL REACTIONS; FUNCTIONS; GENES; MAMMALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; RODENTS; SACCHARIDES; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology