Slow repair of x-ray-induced DNA damage in rat 9L cells in vitro analyzed by viscoelastometry
DNA damage and repair were measured in rat 9L brain tumor cells in vitro using viscoelastometry. Cells were irradiated in saline (no repair) or medium (with repair) and lysed. Following lysis, the viscoelastic retardation time, tau, was determined. In experiments without repair, tau showed an initial increase (0 to 200 rad ..gamma.. rays) followed by a sharp decrease (200 to 1000 rad). When cells were exposed to 1000 rad x rays and allowed postirradiation incubation prior to lysis, the retardation time showed a similar maximum as a function of repair time. These results are discussed in terms of formation and repair of single-strand breaks in conformationally constrained chromosomal DNA, in analogy to results obtained in alkaline sucrose gradient sedimentation studies.
- Research Organization:
- Univ. of California, San Francisco
- OSTI ID:
- 6599059
- Journal Information:
- Radiat. Res.; (United States), Vol. 85:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOLOGICAL REPAIR
TIME DEPENDENCE
DNA
BIOLOGICAL RADIATION EFFECTS
STRAND BREAKS
RADIOINDUCTION
TUMOR CELLS
IN VITRO
RATS
SEDIMENTATION
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)