Diurnal patterns in brain biogenic amines of rats exposed to 60-Hz electric fields
Levels of brain neurotransmitters and their metabolites, as well as concentrations of enzymes associated with their synthesis and metabolism, fluctuate during the day in patterns defined as circadian. The present study examined these rhythms in albino rats exposed to 60-Hz electric fields. Thirty-six animals were exposed to a 39 kV/m field for 4 weeks, 20 h/day, in a parallel-plate electrode system. A group of 36 sham animals was similarly handled and housed in a nonenergized exposure system. On the sampling day, animals were sacrificed at 4-h intervals throughout the 24-h day. Brains were removed, dissected, and kept frozen until chemically analyzed. The levels of biogenic amines and their acidic metabolites in the striatum, hypothalamus, and hippocampus were determined by high-performance liquid chromatography with electrochemical detection (HPLC-ECD) methods. Repeated exposure to 60-Hz electric fields produced significant alterations in the diurnal rhythms of several biogenic amines: dihydroxyphenylacetic acid (DOPAC, the primary metabolite of dopamine in the rat) in the striatum, and norepinephrine, dopamine, and 5-hydroxyindoleacetic acid (5-HIAA; serotonin metabolite) in the hypothalamus. Levels of serotonin in the striatum and hypothalamus showed clear circadian patterns that was not affected by the field. No diurnal or field-related changes were observed in the hippocampal amines.
- Research Organization:
- Loma Linda Univ., CA (USA)
- OSTI ID:
- 6596181
- Journal Information:
- Bioelectromagnetics (N.Y.); (United States), Vol. 9:3
- Country of Publication:
- United States
- Language:
- English
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AMINES
METABOLISM
ELECTROMAGNETIC FIELDS
BIOLOGICAL EFFECTS
BRAIN
DAILY VARIATIONS
HIPPOCAMPUS
HYPOTHALAMUS
LIQUID COLUMN CHROMATOGRAPHY
METABOLITES
RATS
ANIMALS
BODY
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RODENTS
SEPARATION PROCESSES
VARIATIONS
VERTEBRATES
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