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Title: Influence of Rhodococcus equi on the respiratory burst of resident alveolar macrophages from horses

Rhodococcus equi is the etiologic agent of a devastating pneumonia of sporadic incidence in foals. The purpose of this study was to evaluate the influence of R. equi on the superoxide anion production, measured spectrophotometrically as the reduction of cytochrome C, and hexose monophosphate shunt activity, measured by /sup 14/CO/sub 2/ liberation from /sup 14/C-1-D-glucose, of alveolar macrophages from horses. Alveolar macrophages were harvested from 6 anesthetized, healthy, light-breed, adult horses by bronchoalveolar lavage. Following a randomized complete block design, the suspension of cells was divided into aliquots of 10/sup 6/ viable alveolar macrophages which were exposed to 1, 10 or 100 g. of opsonized R. equi or opsonized zymosan A at 37 C for 2 hours. In this study the respiratory burst of equine alveolar macrophages was only evidenced by the hexose monophosphate shunt activity and superoxide anion was not coincidentally produced. Rhodococcus equi did not adversely affect that response. The insignificant superoxide anion production by the alveolar macrophages suggests that this may not be a significant oxygen metabolite in those cells.
Authors:
Publication Date:
OSTI Identifier:
6586467
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D)
Publisher:
Univ. of Illinois,Urbana, IL
Research Org:
Illinois Univ., Urbana (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HORSES; PNEUMONIA; MACROPHAGES; BIOLOGICAL FUNCTIONS; ETIOLOGY; SUPEROXIDE RADICALS; BIOSYNTHESIS; BACTERIAL DISEASES; CARBON 14 COMPOUNDS; GLUCOSE; LAVAGE; LUNGS; RESPIRATION; TRACER TECHNIQUES; ALDEHYDES; ANIMAL CELLS; ANIMALS; BODY; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; DISEASES; FUNCTIONS; HEXOSES; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MONOSACCHARIDES; ORGANIC COMPOUNDS; ORGANS; PHAGOCYTES; RADICALS; RESPIRATORY SYSTEM; RESPIRATORY SYSTEM DISEASES; SACCHARIDES; SOMATIC CELLS; SYNTHESIS; VERTEBRATES 550901* -- Pathology-- Tracer Techniques