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Title: Oxidative product formation in irradiated neutrophils. A flow cytometric analysis

The effect of irradiation on neutrophil oxidative function was evaluated using a flow cytometric assay of intracellular hydrogen peroxide (H/sub 2/O/sub 2/) production. This assay quantitates the H/sub 2/O/sub 2/-dependent conversion of the nonfluorescent compound, 2'-7'-dichlorofluorescein (DCFH), into fluorescent 2'-7'-dichlorofluorescein (DCF) on a single-cell basis. Intracellular H/sub 2/O/sub 2/ production in response to stimulation with phorbol myristate acetate was not affected by neutrophil irradiation at doses up to 2500 rad. In addition, irradiation of intracellular DCFH and aqueous 2'-7'-dichlorofluorescein diacetate (DCFH-DA) resulted in DCF production, which suggested that oxidative molecules produced by aqueous radiolysis were detected by this assay. This study indicates that radiation doses of 1500 to 2500 rad, which are sufficient to prevent induction of graft-versus-host disease by transfused blood components, are not deleterious to neutrophil oxidative metabolism.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
6586370
Resource Type:
Journal Article
Resource Relation:
Journal Name: Transfusion (Philadelphia); (United States); Journal Volume: 2
Research Org:
Univ. of Michigan Medical School, Ann Arbor
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; HYDROGEN PEROXIDE; BIOSYNTHESIS; NEUTROPHILS; BIOLOGICAL FUNCTIONS; BIOLOGICAL RADIATION EFFECTS; PHORBOL ESTERS; BIOLOGICAL EFFECTS; CELL FLOW SYSTEMS; FLUORESCEIN; FLUORESCENCE; GRAFT-HOST REACTION; RADIATION DOSES; TRANSFUSIONS; AROMATICS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CARCINOGENS; DOSES; DYES; ESTERS; FUNCTIONS; HYDROGEN COMPOUNDS; HYDROXY ACIDS; HYDROXY COMPOUNDS; LEUKOCYTES; LUMINESCENCE; MATERIALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PEROXIDES; PHENOLS; POLYPHENOLS; RADIATION EFFECTS; SYNTHESIS; THERAPY 560120* -- Radiation Effects on Biochemicals, Cells, & Tissue Culture; 560300 -- Chemicals Metabolism & Toxicology