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Title: Suppressor cell function is preserved in pemphigus and pemphigoid

Abstract

Human peripheral blood lymphocytes (PBL) are activated to become suppressor T cells (S-T-C) by incubation with Concanavalin-A (Con-A). This has become the standard method for evaluation of suppressor function in patients. S-T-C function has been found to be impaired in several autoimmune diseases, including systemic lupus erythematosus (SLE). Using this assay, we have investigated suppressor-cell function in 2 autoimmune disorders, bullous pemphigoid (BP) and pemphigus vulgaris (PV), studying 6 patients from each group. Three patients with active SLE (positive controls), and 11 normal donors (negative controls) were also included. None of these patients had received systemic therapy with the exception of 2 patients with PV who were treated with gold in the past. PBL from these patients were incubated with and without 40 micrograms/ml Con-A for 72 hr to generate suppressor cells. Both groups of PBL were then irradiated wih 1500 r cobalt. Co-cultures were set up in sextuplicate using normal PBL as responders. Responder PBL were stimulated with 0.5, 1.0, and 2.0 micrograms/ml of phytohemagglutin (PHA) and 5.0, 10.0, and 20.0 micrograms/ml of Con-A. Cultures were pulsed on day 3 with /sup 3/H-thymidine and harvested on day 4. Data were analyzed using Student's t-test. S-T-C function was found tomore » be significantly impaired in SLE vs normal (p . 0.0316). No statistically significant difference was seen in BP (p . 0.5883) and PV (p . 0.0921) as compared with normals. A defect in suppressor cell function may still be present in patients with PV and BP for the defect may be antigen-specific and therefore remain undetected by the Con-A suppressor assay.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Department of Dermatology, University of Michigan Medical School, Ann Arbor
OSTI Identifier:
6579767
Resource Type:
Journal Article
Journal Name:
J. Invest. Dermatol.; (United States)
Additional Journal Information:
Journal Volume: 79:3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; IMMUNE REACTIONS; PATHOLOGY; LUPUS; LYMPHOCYTES; BIOLOGICAL RADIATION EFFECTS; COBALT; CONCANAVALIN; EXTERNAL IRRADIATION; LEUKOCYTES; PATIENTS; AGGLUTININS; ANIMAL CELLS; ANTIBODIES; BACTERIAL DISEASES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; ELEMENTS; HEMAGGLUTININS; INFECTIOUS DISEASES; IRRADIATION; MATERIALS; METALS; RADIATION EFFECTS; SKIN DISEASES; SOMATIC CELLS; TRANSITION ELEMENTS; TUBERCULOSIS; 560151* - Radiation Effects on Animals- Man; 550900 - Pathology; 551000 - Physiological Systems

Citation Formats

King, A J, Schwartz, S A, Lopatin, D, Voorhees, J J, and Diaz, L A. Suppressor cell function is preserved in pemphigus and pemphigoid. United States: N. p., 1982. Web. doi:10.1111/1523-1747.ep12500052.
King, A J, Schwartz, S A, Lopatin, D, Voorhees, J J, & Diaz, L A. Suppressor cell function is preserved in pemphigus and pemphigoid. United States. https://doi.org/10.1111/1523-1747.ep12500052
King, A J, Schwartz, S A, Lopatin, D, Voorhees, J J, and Diaz, L A. 1982. "Suppressor cell function is preserved in pemphigus and pemphigoid". United States. https://doi.org/10.1111/1523-1747.ep12500052.
@article{osti_6579767,
title = {Suppressor cell function is preserved in pemphigus and pemphigoid},
author = {King, A J and Schwartz, S A and Lopatin, D and Voorhees, J J and Diaz, L A},
abstractNote = {Human peripheral blood lymphocytes (PBL) are activated to become suppressor T cells (S-T-C) by incubation with Concanavalin-A (Con-A). This has become the standard method for evaluation of suppressor function in patients. S-T-C function has been found to be impaired in several autoimmune diseases, including systemic lupus erythematosus (SLE). Using this assay, we have investigated suppressor-cell function in 2 autoimmune disorders, bullous pemphigoid (BP) and pemphigus vulgaris (PV), studying 6 patients from each group. Three patients with active SLE (positive controls), and 11 normal donors (negative controls) were also included. None of these patients had received systemic therapy with the exception of 2 patients with PV who were treated with gold in the past. PBL from these patients were incubated with and without 40 micrograms/ml Con-A for 72 hr to generate suppressor cells. Both groups of PBL were then irradiated wih 1500 r cobalt. Co-cultures were set up in sextuplicate using normal PBL as responders. Responder PBL were stimulated with 0.5, 1.0, and 2.0 micrograms/ml of phytohemagglutin (PHA) and 5.0, 10.0, and 20.0 micrograms/ml of Con-A. Cultures were pulsed on day 3 with /sup 3/H-thymidine and harvested on day 4. Data were analyzed using Student's t-test. S-T-C function was found to be significantly impaired in SLE vs normal (p . 0.0316). No statistically significant difference was seen in BP (p . 0.5883) and PV (p . 0.0921) as compared with normals. A defect in suppressor cell function may still be present in patients with PV and BP for the defect may be antigen-specific and therefore remain undetected by the Con-A suppressor assay.},
doi = {10.1111/1523-1747.ep12500052},
url = {https://www.osti.gov/biblio/6579767}, journal = {J. Invest. Dermatol.; (United States)},
number = ,
volume = 79:3,
place = {United States},
year = {Wed Sep 01 00:00:00 EDT 1982},
month = {Wed Sep 01 00:00:00 EDT 1982}
}