Nature and mechanism of induction of mutations. Annual progress report, October 1, 1981-August 1, 1982

Technical Report · Sun Aug 01 00:00:00 EDT 1982

OSTI ID:6571271

Nilan, R A; Kleinhofs, A; Konzak, C F

The major and most significant accomplishment during the past contract year was the chemical identification of the azide metabolite. This accomplishment is unique in plant and bacterial mutagenesis. This is the first observation of a mutagenic amino acid analog. Experiments using barley and bacterial O-acetylserine sulfhydrylase resulted in production of the azide metabolite in vitro. Progress towards understanding the nature of azide-induced mutations continues to be made. In barley (Hordeum vulgare L., cv. Himalaya), DNA-protein crosslinks were detected after azide treatment.

• Research Organization: Washington State Univ., Pullman (USA)
• DOE Contract Number: AT06-76EV72002
• OSTI ID: 6571271
• Report Number(s): DOE/EV/72002-58; ON: DE83004911
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZIDES
METABOLITES
BIOSYNTHESIS
MUTAGENS
BIOLOGICAL PATHWAYS
BARLEY
ENZYME ACTIVITY
IN VITRO
MASS SPECTROSCOPY
SALMONELLA TYPHIMURIUM
SODIUM COMPOUNDS
ALKALI METAL COMPOUNDS
BACTERIA
CEREALS
GRAMINEAE
GRASS
MICROORGANISMS
NITROGEN COMPOUNDS
PLANTS
SALMONELLA
SPECTROSCOPY
SYNTHESIS
550200* - Biochemistry
560303 - Chemicals Metabolism & Toxicology- Plants- (-1987)