Developmental and radiobiologic characteristics of canine multinucleated, osteoclast-like cells generated in vitro from canine bone marrow

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Scan. Microsc.; (United States)

OSTI ID:6565897

Seed, T M; Kaspar, L V; Domann, F; Niiro, G K; LeBuis, D A

We report here our initial observations on the growth and morphology, and developmental radiosensitivity of giant, multinucleated, osteoclast-like cells (MN-OS) generated through in vitro cultivation of hematopoietic progenitor-enriched canine bone marrow samples. Maximum cell densities of 5.5 x 10(3) to 6.5 x 10(3) MN-OS per cm2 of growth area were achieved following 10 to 14 days of culture at 37 degrees C. Acute gamma irradiation of the initial marrow inocula resulted in significant, dose-dependent perturbations of MN-OS formation, growth, and development. Attempts to estimate radiosensitivity of MN-OS progenitors from canine marrow yielded a range of Do values from a low of 212 cGy measured at six days of culture to higher values of 405 to 542 cGy following 10 to 22 days of culture. At the intermediate times of culture (10 to 14 days), the radiation-induced responses were clearly biphasic, reflecting either (a) the presence of multiple subpopulations of MN-OS progenitors with varying degrees of radiosensitivity or (b) the inherent biphasic nature of MN-OS development involving early progenitor cell proliferation followed by maturation and subsequent fusion. Morphologically, MN-OS generated from irradiated marrow inocula appeared only marginally altered, with alterations expressed largely in a biphasic, dose-dependent fashion in terms of smaller cell size, reduced number of nuclei, increased expression of both surface microprojections, and a unique set of crystalloid cytoplasmic inclusions. Functionally, MN-OS appeared to be impaired by irradiation of marrow progenitors, as evidenced by failure to initiate resorptive attachments to devitalized bone spicules in vitro.

• Research Organization: Argonne National Lab,, IL (USA)
• OSTI ID: 6565897
• Journal Information: Scan. Microsc.; (United States), Vol. 2:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
RADIOSENSITIVITY
BIOLOGICAL RADIATION EFFECTS
BONE MARROW
CELL CULTURES
CELL DIVISION
CELL PROLIFERATION
DOGS
DOSE-RESPONSE RELATIONSHIPS
GAMMA RADIATION
RADIOBIOLOGY
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGY
BODY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
HEMATOPOIETIC SYSTEM
IONIZING RADIATIONS
MAMMALS
ORGANS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture