Kidney allograft survival in dogs treated with total lymphoid irradiation
Total lymphoid irradiation (TLI) is immunosuppressive and, in rodents, can induce a state where transplantation of allogenic bone marrow results in chimerism and permanent acceptance of organ allografts from the donor strain. Twelve splenectomized dogs were treated with TLI (150 rads per fraction, total dose 1950 to 3000 rads) before bilateral nephrectomy and renal allotransplantation. Eight dogs received bone marrow from the kidney donor. In 13 untreated control dogs renal allografts functioned for a mean +- (SE) of 4.7 +- 0.3 days. In the four TLI treated dogs who did not receive bone marrow the renal allografts functioned for 15 to 76 days (two dogs died with functioning grafts). In the eight TLI treated dogs who received donor bone marrow, two died immediately after transplantation, two rejected at 3 and 13 days, one died at 13 days with a functioning graft, and two have had the grafts function for longer than 500 days. Chimerism was not detected in the one dog tested. The response of peripheral blood lymphocytes to stimulation with phytohemaglutinin and in mixed lymphocyte culture was suppressed for at least one month after TLI. The results confirm the immunosuppressive effect of TLI. The absence of kidney rejection in two recipients of donor bone marrow show the potential of this approach to induce long-term immunologic unresponsiveness as to an organ allograft, but the outcome is unpredictable and further experiments are needed to define the optimal conditions for administration of TLI and bone marrow to the recipients.
- Research Organization:
- Univ. of Minnesota, Minneapolis, MN (United States)
- OSTI ID:
- 6564743
- Journal Information:
- Ann. Surg.; (United States), Vol. 193:2
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
IMMUNE REACTIONS
BIOLOGICAL RADIATION EFFECTS
IMMUNOSUPPRESSION
RADIOINDUCTION
BEAGLES
BONE MARROW CELLS
KIDNEYS
LINEAR ACCELERATORS
LYMPHATIC SYSTEM
MEV RANGE 01-10
PARTIAL BODY IRRADIATION
SPLENECTOMY
TRANSPLANTS
ACCELERATORS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BODY
CONNECTIVE TISSUE CELLS
DOGS
ENERGY RANGE
EXTERNAL IRRADIATION
IRRADIATION
MAMMALS
MEDICINE
MEV RANGE
ORGANS
RADIATION EFFECTS
SOMATIC CELLS
SURGERY
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
550600 - Medicine
551000 - Physiological Systems