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Title: Properties of cellulase immobilized on agarose gel with spacer

Cellulase produced by fungus Trichoderma viride was immobilized on agarose beads (Sepharose 4B) activated by cyanogen bromide and also on activated agarose beads that contained spacer arm (activated Ch-Sepharose 4B and Affi-Gel 15). The CMCase activity retained by immobilized cellulase on activated Sepharose containing the spacer tended to be higher than that immobilized without spacer, although the extent of protein immobilization was lower. Also, the higher substrate specificity for cellulase immobilized on beads with spacer was obtained for cellobiose, acid-swollen cellulose, or cellulose powder. The hydrolysis product from their substrates was mainly glucose. 10 references.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
6561637
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biotechnol. Bioeng.; (United States); Journal Volume: 28:12
Research Org:
National Food Research Institute, Ibaraki, Japan
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; CELLULASE; ENZYME ACTIVITY; IMMOBILIZED ENZYMES; AGAR; CELLOBIOSE; CHEMICAL ACTIVATION; ENZYMATIC HYDROLYSIS; GELS; GLUCOSE; PH VALUE; POWDERS; PRODUCTION; PROTEINS; SUBSTRATES; TRICHODERMA VIRIDE; ALDEHYDES; CARBOHYDRATES; CHEMICAL REACTIONS; COLLOIDS; DECOMPOSITION; DISACCHARIDES; DISPERSIONS; ENZYMES; FUNGI; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROLYSIS; LYSIS; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; TRICHODERMA 090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989); 550700 -- Microbiology; 140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)