Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method
Abstract
Direct-drug-susceptibility tests were performed on clinical specimens positive for acid-fast bacilli by either Ziehl-Neelsen or fluorochrome staining. The results of conventional agar dilution and a modified radiometric (BACTEC) method were compared. A total of 580 smear-positive specimens were tested by the BACTEC method at three separate sites. Three hundred and seventy-seven of these were culture positive for M. tuberculosis, and 343 (91%) yielded acceptable direct-susceptibility-test results. We used the conventional method to determine that 343 of 519 smear-positive specimens were culture positive for M. tuberculosis, and 212 (62%) produced acceptable results within 3 wks. Conventional results were reported in 3-4 wks, while the time required to obtain results with the BACTEC method ranged from 5 to 21 days (average 11.5 days). Results indicate that the radiometric method provides reportable results more frequently with time savings as compared to the conventional method.
- Authors:
- Publication Date:
- Research Org.:
- Maryland Department of Health and Mental Hygiene, Baltimore (USA)
- OSTI Identifier:
- 6560367
- Resource Type:
- Journal Article
- Journal Name:
- Diagn. Microbiol. Infect. Dis.; (United States)
- Additional Journal Information:
- Journal Volume: 10:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; BACTERIAL DISEASES; DIAGNOSIS; MYCOBACTERIUM TUBERCULOSIS; RADIOMETRIC ANALYSIS; ISONIAZID; STREPTOMYCIN; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTIMICROBIAL AGENTS; BACTERIA; CHEMICAL ANALYSIS; DISEASES; DRUGS; HYDRAZIDES; INFECTIOUS DISEASES; MICROORGANISMS; MYCOBACTERIUM; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; QUANTITATIVE CHEMICAL ANALYSIS; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550901 - Pathology- Tracer Techniques
Citation Formats
Libonati, J P, Stager, C E, Davis, J R, and Siddiqi, S H. Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method. United States: N. p., 1988.
Web. doi:10.1016/0732-8893(88)90125-3.
Libonati, J P, Stager, C E, Davis, J R, & Siddiqi, S H. Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method. United States. https://doi.org/10.1016/0732-8893(88)90125-3
Libonati, J P, Stager, C E, Davis, J R, and Siddiqi, S H. 1988.
"Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method". United States. https://doi.org/10.1016/0732-8893(88)90125-3.
@article{osti_6560367,
title = {Direct antimicrobial drug susceptibility testing of Mycobacterium tuberculosis by the radiometric method},
author = {Libonati, J P and Stager, C E and Davis, J R and Siddiqi, S H},
abstractNote = {Direct-drug-susceptibility tests were performed on clinical specimens positive for acid-fast bacilli by either Ziehl-Neelsen or fluorochrome staining. The results of conventional agar dilution and a modified radiometric (BACTEC) method were compared. A total of 580 smear-positive specimens were tested by the BACTEC method at three separate sites. Three hundred and seventy-seven of these were culture positive for M. tuberculosis, and 343 (91%) yielded acceptable direct-susceptibility-test results. We used the conventional method to determine that 343 of 519 smear-positive specimens were culture positive for M. tuberculosis, and 212 (62%) produced acceptable results within 3 wks. Conventional results were reported in 3-4 wks, while the time required to obtain results with the BACTEC method ranged from 5 to 21 days (average 11.5 days). Results indicate that the radiometric method provides reportable results more frequently with time savings as compared to the conventional method.},
doi = {10.1016/0732-8893(88)90125-3},
url = {https://www.osti.gov/biblio/6560367},
journal = {Diagn. Microbiol. Infect. Dis.; (United States)},
number = ,
volume = 10:1,
place = {United States},
year = {Sun May 01 00:00:00 EDT 1988},
month = {Sun May 01 00:00:00 EDT 1988}
}