A simulation model for the continuous production of acetoin and butanediol using Bacillus subtilis with integrated pervaporation separation

Journal Article · Thu Apr 01 00:00:00 EST 1993 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260410805· OSTI ID:6546799

Dettwiler, B; Dunn, I J; Heinzle, E; Prenosil, J E ^[1]

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1. ETH, Zurich (Switzerland)

The potential for producing acetoin and butanediol with a Bacillus subtilis strain was investigated with continuous culture using molasses as carbon substrate. The steady-state results were influenced by both oxygen and undetermined limiting compounds. Employing the known metabolic pathways, four overall stoichiometric relations were used with an energetic assumption on the energy requirements for biomass formation to establish a linear relation between the overall rates, whose parameters were determined by linear regression. This provided a relationship for the product formation rate. The chemostat culture data were described with a growth kinetics model, which included limitation by molasses and oxygen as well as diauxic effects and product inhibition. The biokinetics model was combined with an experimentally verified model for the membrane pervaporation. From this combined model were determined the influence of the membrane characteristics (enrichment factors and membrane area) and the dilution rate on the performance of the integrated process. Simulations revealed that an increase of the enrichment factor, possible by membrane improvement, would have counteracting influences, owing to decreased product inhibition but with lower biomass concentration.

• OSTI ID: 6546799
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 41:8; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
BACILLUS SUBTILIS
GROWTH
BUTANEDIOLS
BIOSYNTHESIS
BIOREACTORS
CONTINUOUS CULTURE
MATHEMATICAL MODELS
METABOLISM
REGRESSION ANALYSIS
STEADY-STATE CONDITIONS
STOICHIOMETRY
ALCOHOLS
BACILLUS
BACTERIA
GLYCOLS
HYDROXY COMPOUNDS
MATHEMATICS
MICROORGANISMS
ORGANIC COMPOUNDS
STATISTICS
SYNTHESIS
090900* - Biomass Fuels- Processing- (1990-)