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Title: Quinones: reactions with hemoglobin, effects within erythrocytes and potential for antimalarial development

Abstract

The focus of this research was to characterize the interactions of some simple quinone like compounds with purified hemoglobin and to study the effects of these compounds within erythrocytes. It is proposed that these sorts of agents can have an antimalarial effect. The simplest compounds chosen for study were benzoquinone, methylquinone (toluquinone) and hydroquinone. When /sup 14/C-quinone was reacted with purified hemoglobin (Hb) there was rapid binding of the first two moles of substrate per Hb molecule. An unusual property of the modified Hb's is that in the presence of a redox sensitive agent such as cytochrome c they are capable of generating superoxide anions. Within erythrocytes, quinone and toluquinone which differ only by a single methyl group have completely different effects. Toluquinone causes the cells to hemolyse and the effect was enhanced when the erythrocyte superoxide dismutase was inhibited; the effect was diminished when scavengers of activated oxygen such as histidine, mannitol and vital E were present. Benzoquinone on the other hand did not cause the cells to hemolyse and instead appeared to protect the cells from certain hemolytic stresses. Growth of malaria parasites in erythrocytes has been shown to be inhibited by activated forms of oxygen, also somemore » quinone like agents in the past have been shown to inhibit the parasite's metabolism. An initial experiment with erythrocytes infected with malaria parasites showed that quinone and toluquinone could both inhibit the growth rate of parasites.« less

Authors:
Publication Date:
Research Org.:
Case Western Reserve Univ., Cleveland, OH (USA)
OSTI Identifier:
6544106
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; ERYTHROCYTES; LYSIS; MALARIA; PATHOGENESIS; PARASITES; GROWTH; QUINONES; BIOLOGICAL EFFECTS; CARBON 14 COMPOUNDS; HEMOGLOBIN; INHIBITION; SUPEROXIDE DISMUTASE; SUPEROXIDE RADICALS; TRACER TECHNIQUES; AROMATICS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; DISEASES; ENZYMES; GLOBIN; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MATERIALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; OXIDOREDUCTASES; PARASITIC DISEASES; PIGMENTS; PORPHYRINS; PROTEINS; RADICALS; 560300* - Chemicals Metabolism & Toxicology; 550901 - Pathology- Tracer Techniques

Citation Formats

Denny, B J. Quinones: reactions with hemoglobin, effects within erythrocytes and potential for antimalarial development. United States: N. p., 1986. Web.
Denny, B J. Quinones: reactions with hemoglobin, effects within erythrocytes and potential for antimalarial development. United States.
Denny, B J. 1986. "Quinones: reactions with hemoglobin, effects within erythrocytes and potential for antimalarial development". United States.
@article{osti_6544106,
title = {Quinones: reactions with hemoglobin, effects within erythrocytes and potential for antimalarial development},
author = {Denny, B J},
abstractNote = {The focus of this research was to characterize the interactions of some simple quinone like compounds with purified hemoglobin and to study the effects of these compounds within erythrocytes. It is proposed that these sorts of agents can have an antimalarial effect. The simplest compounds chosen for study were benzoquinone, methylquinone (toluquinone) and hydroquinone. When /sup 14/C-quinone was reacted with purified hemoglobin (Hb) there was rapid binding of the first two moles of substrate per Hb molecule. An unusual property of the modified Hb's is that in the presence of a redox sensitive agent such as cytochrome c they are capable of generating superoxide anions. Within erythrocytes, quinone and toluquinone which differ only by a single methyl group have completely different effects. Toluquinone causes the cells to hemolyse and the effect was enhanced when the erythrocyte superoxide dismutase was inhibited; the effect was diminished when scavengers of activated oxygen such as histidine, mannitol and vital E were present. Benzoquinone on the other hand did not cause the cells to hemolyse and instead appeared to protect the cells from certain hemolytic stresses. Growth of malaria parasites in erythrocytes has been shown to be inhibited by activated forms of oxygen, also some quinone like agents in the past have been shown to inhibit the parasite's metabolism. An initial experiment with erythrocytes infected with malaria parasites showed that quinone and toluquinone could both inhibit the growth rate of parasites.},
doi = {},
url = {https://www.osti.gov/biblio/6544106}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}

Thesis/Dissertation:
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