Opioid-dependent growth of glial cultures: Suppression of astrocyte DNA synthesis by met-enkephalin

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90041-O· OSTI ID:6532939

Stiene-Martin, A; Hauser, K F ^[1]

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1. Univ. of Kentucky, Lexington (USA)

The action of met-enkephalin on the growth of astrocytes in mixed-glial cultures was examined. Primary, mixed-glial cultures were isolated from 1 day-old mouse cerebral hemispheres and continuously treated with either basal growth media, 1 {mu}M met-enkephalin, 1 {mu}M met-enkephalin plus the opioid antagonist naloxone, or naloxone alone. Absolute numbers of neural cells were counted in unstained preparations, while combined ({sup 3}H)-thymidine autoradiography and glial fibrillary acid protein (GFAP) immunocytochemistry was performed to identify specific changes in astrocytes. When compared to control and naloxone treated cultures, met-enkephalin caused a significant decrease in both total cell numbers, and in ({sup 3}H)-thymidine incorporation by GFAP-positive cells with flat morphology. These results indicate that met-enkephalin suppresses astrocyte growth in culture.

• OSTI ID: 6532939
• Journal Information: Life Sciences; (USA), Vol. 46:2; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENKEPHALINS
BIOLOGICAL EFFECTS
NERVE CELLS
GROWTH
ANTIMETABOLITES
AUTORADIOGRAPHY
BRAIN
DNA REPLICATION
INHIBITION
MICE
NEUROREGULATORS
THYMIDINE
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZINES
BODY
CENTRAL NERVOUS SYSTEM
DRUGS
ENDORPHINS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
MAMMALS
NERVOUS SYSTEM
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques