Molecular control of the induction of alcohol dehydrogenase by ethanol in Drosophila melanogaster larvae

Journal Article · Sun Apr 01 00:00:00 EST 1990 · Genetics; (USA)

OSTI ID:6532852

Kapoun, A M; Geer, B W; Heinstra, P W.H. ^[1]; Corbin, V ^[2]; McKechnie, S W ^[3]

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1. Knox College, Galesburg, IL (USA)
2. Harvard Univ., Cambridge, MA (USA)
3. Monash Univ., Victoria (Australia)

The activity of alcohol dehydrogenase, the initial enzyme in the major pathway for ethanol degradation, is induced in Drosophila melanogaster larvae by low concentrations of dietary ethanol. Two lines of evidence indicate that the metabolic products of the ADH pathway for ethanol degradation are not directly involved in the induction of Adh. First, the accumulation of the proximal transcript in Adh{sup n2} larvae was increased when the intracellular level of ethanol was elevated. In addition, the ADH activity, the proximal Adh mRNA, and the intracellular concentration of ethanol were elevated coordinately in wild-type larvae fed hexadeuterated-ethanol, which is metabolized more slowly than normal ethanol.l An examination of P element transformant lines with specific deletions in the 5{prime} regulatory DNA of the Adh gene showed that the DNA sequence between +604 and +634 of the start site of transcription from the distal promoter was essential for this induction. The DNA sequence between {minus}660 and about {minus}5,000 of the distal transcript start site was important for the down-regulation of the induction response.

• OSTI ID: 6532852
• Journal Information: Genetics; (USA), Vol. 124:4; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALCOHOL DEHYDROGENASE
ENZYME INDUCTION
BIOLOGICAL PATHWAYS
DEUTERIUM COMPOUNDS
DROSOPHILA
GENE REGULATION
LARVAE
PHOSPHORUS 32
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIPTERA
ENZYMES
FLIES
FRUIT FLIES
HEMIACETAL DEHYDROGENASES
HYDROGEN COMPOUNDS
INSECTS
INVERTEBRATES
ISOTOPES
LIGHT NUCLEI
NUCLEI
ODD-ODD NUCLEI
OXIDOREDUCTASES
PHOSPHORUS ISOTOPES
RADIOISOTOPES
550201* - Biochemistry- Tracer Techniques