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Title: Regulation and ontogeny of subtypes of muscarinic receptors and muscarinic receptor-mediated

The densities of total and M1 muscarinic receptors were measured using the muscarinic receptor antagonists {sup 3}H-quinuclidinyl benzilate and {sup 3}H-pirenzepine, respectively. Thus, the difference between the density of {sup 3}H-quinuclidinyl benzilate and {sup 3}H-pirenzepine binding sites represents the density of M2 sites. In addition, there is no observable change in either acetylcholine-stimulated phosphoinositide breakdown (suggested to be an M1 receptor-mediated response) or in carbachol-mediated inhibition of cyclic AMP accumulation (suggested to be an M2 receptor-mediated response) in slices of cortex+dorsal hippocampus following chronic atropine administration. In other experiments, it has been shown that the M1 and M2 receptors in rat cortex have different ontogenetic profiles. The M2 receptor is present at adult levels at birth, while the M1 receptor develops slowly from low levels at postnatal week 1 to adult levels at postnatal week 3. The expression of acetylcholine-stimulated phosphoinositide breakdown parallels the development of M1 receptors, while the development of carbachol-mediated inhibition of cyclic AMP accumulation occurs abruptly between weeks 2 and 3 postnatally.
Authors:
Publication Date:
OSTI Identifier:
6531761
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
Philadelphia, PA (US); Univ. of Pennsylvania
Research Org:
Pennsylvania Univ., Philadelphia, PA (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMP; BIOLOGICAL ACCUMULATION; PARASYMPATHOMIMETICS; RECEPTORS; PHOSPHOLIPIDS; METABOLISM; ONTOGENESIS; ACETYLCHOLINE; BIOCHEMICAL REACTION KINETICS; CEREBRAL CORTEX; HIPPOCAMPUS; PARASYMPATHOLYTICS; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; AMINES; AMMONIUM COMPOUNDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; BRAIN; CENTRAL NERVOUS SYSTEM; CEREBRUM; DRUGS; ESTERS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; LIPIDS; MAMMALS; MEMBRANE PROTEINS; NERVOUS SYSTEM; NEUROREGULATORS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; PROTEINS; QUATERNARY COMPOUNDS; REACTION KINETICS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques