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Title: Specific fixation of bovine brain and retinal acidic and basic fibroblast growth factors to mouse embryonic eye basement membranes

The labeling pattern of mouse embryonic eye frozen sections incubated with radioiodinated brain acidic and basic fibroblasts growth factors (aFGF and bFGF) was investigated by autoradiography. Both growth factors bind to basement membranes in a dose-dependent way, with a higher affinity for bFGF. Similar data were obtained with eye-derived growth factors (EDGF), the retinal forms of FGF. There was a heterogeneity in the affinity of the various basement membranes toward these growth factors. The specificity of the growth factor-basement membrane interaction was demonstrated by the following experiments: (i) an excess of unlabeled growth factor displaced the labeling; (ii) unrelated proteins with different isoelectric points did not modify the labeling; and (iii) iodinated EGF or PDGF did not label basement membrane. In order to get a better understanding of the nature of this binding, the authors performed the incubation of the frozen sections with iodinated FGFs preincubated with various compounds. These results demonstrate that FGFs bind specifically to basement membranes, probably on the polysaccharidic part of the proteoheparan sulfate, and suggest that this type of interaction may be a general feature of the mechanism of action of these growth factors.
Authors:
; ;  [1] ; ; ;  [2]
  1. (INSERM U.118, Paris (France))
  2. (Universite Paris Val de Marne, Creteil (France))
Publication Date:
OSTI Identifier:
6531637
Resource Type:
Journal Article
Resource Relation:
Journal Name: Experimental Cell Research; (USA); Journal Volume: 171:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FETAL MEMBRANES; CROSS-LINKING; GROWTH FACTORS; RADIORECEPTOR ASSAY; POLYPEPTIDES; BRAIN; CATTLE; ELECTROPHORESIS; EMBRYOS; EYES; FIBROBLASTS; IODINE 125; MICE; RETINA; SODIUM IODIDES; ALKALI METAL COMPOUNDS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; BODY AREAS; CENTRAL NERVOUS SYSTEM; CHEMICAL REACTIONS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; FACE; HALIDES; HALOGEN COMPOUNDS; HEAD; INORGANIC PHOSPHORS; INTERMEDIATE MASS NUCLEI; IODIDES; IODINE COMPOUNDS; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEMBRANES; MITOGENS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDES; PHOSPHORS; POLYMERIZATION; PROTEINS; RADIOISOTOPES; RODENTS; RUMINANTS; SENSE ORGANS; SODIUM COMPOUNDS; SOMATIC CELLS; TRACER TECHNIQUES; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques