Interleukin-4 receptors on human blood mononuclear cells
- Laboratory for Immunological Research, Dardilly (France)
We have studied regulation of the expression of the interleukin-4 receptor (IL-4R) on human blood mononuclear cells (PBMC) using both 125I-IL-4 binding assay and flow cytometric analysis of biotinylated IL-4 (B-IL-4) binding. PBMC express approximately 300 high-affinity IL-4R per cell (Kd = 25-100 pM). Activation of PBMC for 60-80 hr by phytohemagglutinin (PHA) or concanavalin A (Con A) results in a 2- to 4.5-fold increase of IL-4R number without alteration of IL-4R affinity for IL-4. Binding of B-IL-4 showed that IL-4R expression is upregulated on virtually all PHA-stimulated PBMC, whereas it mostly concerns larger cells among Con A-activated PBMC. Reculture of PHA-blasts with 1 nM IL-4 further upregulates IL-4R expression to a level approximately 10-fold higher than observed on freshly isolated PBMC. Interestingly, IL-4 is able to reinduce high IL-4R levels on cells that have been deprived of IL-4 for 20 hr and IL-2 is almost as efficient. Finally, SDS-PAGE analysis of IL-4-binding molecules on unstimulated, PHA- and PHA/IL-4-activated PBMC revealed the same three peptides of MW 140-130, 80-75, and 70-65 kDa, as shown on human cell lines.
- OSTI ID:
- 6529939
- Journal Information:
- Cellular Immunology; (USA), Vol. 129:2; ISSN 0008-8749
- Country of Publication:
- United States
- Language:
- English
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LYMPHOKINES
RECEPTORS
GENE REGULATION
BIOCHEMICAL REACTION KINETICS
CELL FLOW SYSTEMS
CONCANAVALIN
ELECTROPHORESIS
IN VITRO
IODINE 125
LYMPHOCYTES
MAN
MONOCYTES
PHYTOHEMAGGLUTININ
RADIOASSAY
TRACER TECHNIQUES
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GROWTH FACTORS
HEMAGGLUTININS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MUCOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques