Use of ( sup 11 C)aminocyclohexanecarboxylate for the measurement of amino acid uptake and distribution volume in human brain

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Journal of Cerebral Blood Flow and Metabolism; (USA)

DOI:https://doi.org/10.1038/jcbfm.1990.126· OSTI ID:6528433

Koeppe, R A; Mangner, T; Betz, A L; Shulkin, B L; Allen, R; Kollros, P; Kuhl, D E; Agranoff, B W ^[1]

---

1. Univ. of Michigan Medical School, Ann Arbor (USA)

A quantitative positron emission tomographic (PET) method to measure amino acid blood-brain barrier (BBB) transport rate and tissue distribution volume (DV) has been developed using {sup 11}C-labeled aminocyclohexanecarboxylate (ACHC), a nonmetabolized amino acid analogue. Dynamic PET data were acquired as a series of 15 scans covering a total of 60 min and analyzed by means of a two-compartment, two-parameter model. Functional images were calculated for the amino acid transport rate constants across the BBB and the amino acid DV in the brain. Results show ({sup 11}C)ACHC to have an influx rate constant in gray matter of approximately 0.03-0.04 ml g-1 min-1, indicating a single-pass extraction fraction of approximately 5-7%. The intersubject coefficient of variation was approximately 15% while intrasubject variability of repeat scans was only slightly greater than 5%. Studies were performed in 15 young normal volunteer control subjects, 5 elderly controls, 7 patients with probable Alzheimer's disease, and one patient with phenylketonuria. Results indicate that ({sup 11}C)-ACHC will serve as the basis of a method for measuring amino acid transport rate and DV in the normal and pathological human brain.

• OSTI ID: 6528433
• Journal Information: Journal of Cerebral Blood Flow and Metabolism; (USA), Vol. 10:5; ISSN 0271-678X
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
MEMBRANE TRANSPORT
BLOOD-BRAIN BARRIER
PERMEABILITY
BRAIN
POSITRON COMPUTED TOMOGRAPHY
NERVOUS SYSTEM DISEASES
DIAGNOSIS
CARBON 11
PATIENTS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CARBON ISOTOPES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
COMPUTERIZED TOMOGRAPHY
DIAGNOSTIC TECHNIQUES
DISEASES
EMISSION COMPUTED TOMOGRAPHY
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
MINUTES LIVING RADIOISOTOPES
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
TOMOGRAPHY
550601* - Medicine- Unsealed Radionuclides in Diagnostics