skip to main content

Title: Uptake and/or utilization of two simple phenolic acids by Cucumis sativus

The uptake of ferulic acid (FA) and p-hydroxybenzoic acid (p-HBA) from solutions (0.1 to 1.00 mM, pH 4.0 to 7.0), was determined for intact and excised roots of Cucumis sativus. Uptake methods based on high performance liquid chromatographic (HPLC) analysis of phenolic acid depletion from solution were compared to those radioisotopic methods employing (U-ring-/sup 14/C)FA or p-HBA. Although radiotracer methods more accurately reflected actual uptake of the compounds by cucumber seedlings, HPLC solution depletion methods may be useful in the elucidation of trends over very limited periods of time. The uptake of FA was unaffected by the presence of p-HBA. The uptake of p-HBA was reduced by 30% in the presence of FA when compared to the uptake from solutions containing p-HBA alone. Ferulic acid acts both as an allelopathic agent and precursor in the endogenous process of lignification. To evaluate the involvement of exogenous FA in lignin biosynthesis, roots of hydroponically grown cucumber seedlings were exposed to concentrations of FA labeled with (U-ring-/sup 14/C)FA. Radiotracer was distributed throughout the seedling. A quantitative change in lignification occurred in treated seedlings. In roots and stems, the level of lignin increased with the number of exposures and as the concentrations of exogenousmore » FA increased. Radiotracer was found in the residues of lignin isolated from seedling tissue treated with (U-ring-/sup 14/C)FA. This suggested the utilization of the exogenously applied FA in the endogenous process of lignification.« less
Authors:
Publication Date:
OSTI Identifier:
6527191
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
North Carolina State Univ.,Raleigh, NC
Research Org:
North Carolina State Univ., Raleigh (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CARBON 14 COMPOUNDS; TISSUE DISTRIBUTION; UPTAKE; CARBOXYLIC ACIDS; CUCUMBERS; LIGNIN; LIQUID COLUMN CHROMATOGRAPHY; ROOTS; SEEDLINGS; CARBOHYDRATES; CHROMATOGRAPHY; DISTRIBUTION; FOOD; LABELLED COMPOUNDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SEPARATION PROCESSES; VEGETABLES 550201* -- Biochemistry-- Tracer Techniques