Nuclear ploidy of neonatal rat livers: effects of two hepatic carcinogens (mirex and dimethylnitrosamine)
The effect of two hepatic carcinogens, dimethylnitrosamine (DMN) (genotoxic) and mirex (epigenetic), on polyploidization in 12-d-old neonatal rats was investigated by Coulter counteranalysis and (/sup 3/H) thymidine uptake in isolated hepatic nuclear classes. DMN disturbed the normal ploidy development in the neonatal liver and the proportion of nuclei in the ploidy classes by inducing the premature formation of a significant population of tetraploids with a concommitant reduction in diploids. A great proportion of the replicative activity was present in tetraploid nuclei as measured by the incorporation of (/sup 3/H) thymidine. The labeling index and number of mitoses were also increased. In contrast to DMN, mirex had no influence on polyploidization. The neonatal rats used in these studies thus offer an opportunity to investigate in vivo the mode of action of genotoxic versus epigenetic compounds with reference to their effect on DNA.
- Research Organization:
- Institute of Experimental Pathology and Toxicology, Albany, NY
- OSTI ID:
- 6524845
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 15:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
LIVER CELLS
PLOIDY
NITROSAMINES
TOXICITY
PESTICIDES
CARCINOGENESIS
EXPERIMENTAL DATA
IN VIVO
MITOSIS
NEONATES
RATS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
AZINES
CELL DIVISION
DATA
HETEROCYCLIC COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NITROSO COMPOUNDS
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOGENESIS
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550301 - Cytology- Tracer Techniques