Mechanistic studies on the phytylation and methylation steps in bacteriochlorophyll a biosynthesis: an application of the /sup 18/O-induced isotope effect in /sup 13/C NMR
The high-resolution /sup 13/C NMR spectrum of bacteriochlorophyll a biosynthesized from (1-/sup 13/C,1,1,4-/sup 18/O/sub 3/)-5-aminolevulinic acid by growing cells of Rhodopseudomonas sphaeroides has shown both the C-17/sup 3/ and C-13/sup 3/ resonances consist of three additional components upfield shifted from the -/sup 16/O- /sup 13/C double bond /sup 16/O resonance. By comparison with the /sup 13/C NMR spectrum obtained for phytyl acetate containing /sup 13/C and /sup 18/O selectively in the ester linkage, these components have been identified as the bridge (-/sup 18/O- /sup 13/C double bond /sup 16/O), non bridge (-/sup 16/O-/sup 13/C double bond /sup 18/O), and dual-labeled (-/sup 18/O-/sup 13/C double bond /sup 18/O) isotopomers, These results have been interpreted to suggest that both the ester bonds of bacteriochlorophyll a are produced by a carboxy-alklyl transfer process.
- Research Organization:
- Univ. of Southampton, England
- OSTI ID:
- 6522100
- Journal Information:
- Biochemistry; (United States), Vol. 26:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
AMINOLEVULINIC ACID
NUCLEAR MAGNETIC RESONANCE
CHLOROPHYLL
BIOSYNTHESIS
LABELLED COMPOUNDS
BIOLOGICAL PATHWAYS
METHYLATION
NMR SPECTRA
OXYGEN 13
RHODOPSEUDOMONAS
SPECTRAL SHIFT
AMINO ACIDS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MICROORGANISMS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN ISOTOPES
PHYTOCHROMES
PIGMENTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
RESONANCE
SPECTRA
SYNTHESIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques