Mutational specificities of environmental carcinogens in the lacl gene of Escherichia coli. III. The cyclic nitrosamine N-nitrosopyrrolidine is a complex mutagen
- York Univ., Toronto, Ontario (Canada)
The mutational specificity of the cyclic nitrosamine N-nitrosopyrrolidine (NPYR) was determined through the DNA sequence characterization of 33 lacl-d mutations of Escherichia coli. Base substitution was the predominant class of mutation induced (91%). The majority of these (64%) occurred at GC base pairs, in accordance with the predicted significance of NPYR-derived guanine adducts. In addition, this nitrosamine efficiently produced other kinds of base substitution events as 11 of the 33 mutations occurred at AT base pairs. Deletion, frameshift, and duplication events were also recovered. The complexity of the NPYR mutational spectrum appears to be consistent with the suggestion that this compound acts through both direct and indirect mutational pathways.
- OSTI ID:
- 6518518
- Journal Information:
- Mol. Carcinog; (USA), Vol. 3:3; ISSN 0899-1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ESCHERICHIA COLI
SENSITIVITY
NITROSAMINES
MUTAGENESIS
BIOLOGICAL PATHWAYS
DNA ADDUCTS
DNA SEQUENCING
GENE MUTATIONS
GENES
GENETIC EFFECTS
ADDUCTS
AMINES
BACTERIA
BIOLOGICAL EFFECTS
MICROORGANISMS
MUTATIONS
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
560300* - Chemicals Metabolism & Toxicology