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Title: Effect of cysteamine on cytosolic somatostatin binding sites in rabbit duodenal mucosa

Administration of cysteamine in rabbits elicited a rapid depletion of both duodenal mucosa and plasma somatostatin. A significant reduction was observed within 5 min, returning toward control values by 150 min. The depletion of somatostatin was associated with an increase in the binding capacity and a decrease in the affinity of both high- and low-affinity binding sites present in cytosol of duodenal mucosa. Incubation of cytosolic fraction from control rabbits with 1 mM cysteamine did not modify somatostatin binding. Furthermore, addition of cysteamine at the time of binding assay did not affect the integrity of /sup 125/I-Tyr11-somatostatin. It is concluded that in vivo administration of cysteamine to rabbits depletes both duodenal mucosa and plasma somatostatin and leads to up-regulation of duodenal somatostatin binding sites.
Authors:
; ; ; ;
Publication Date:
OSTI Identifier:
6518429
Resource Type:
Journal Article
Resource Relation:
Journal Name: Exp. Mol. Pathol.; (United States); Journal Volume: 2
Research Org:
Universidad de Alcala de Henares, Madrid, Spain
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MEA; TOXICITY; SOMATOSTATIN; BIOCHEMICAL REACTION KINETICS; IODINE 125; IODINE COMPOUNDS; MUCOUS MEMBRANES; RABBITS; SMALL INTESTINE; TRACER TECHNIQUES; AMINES; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; GASTROINTESTINAL TRACT; HALOGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTESTINES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MEMBRANES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; ORGANS; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; REACTION KINETICS; THIOLS; VERTEBRATES 560151* -- Radiation Effects on Animals-- Man