Enhanced phagocytosis by alveolar macrophages induced by short-term ozone insult

Journal Article · Sun Aug 01 00:00:00 EDT 1982 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(82)90127-X· OSTI ID:6514069

Christman, C A; Schwartz, L W

In vitro phagocytosis of inert microspheres by the alveolar macrophage (AM) was evaluated after in vivo exposure to 0.8 ppm ozone for 3, 7, or 20 days. AM were collected by bronchoalveolar lavage from rats, allowed to adhere to glass, and incubated with carbon-coated latex microspheres. The percentages of phagocytic cells were determined by light microscopy after 0.25, 0.5, 1, 2, 4, 8, and 24 hr of incubation. Morphological features of AM were evaluated by scanning electron microscopy (SEM) and an index of cell spreading was determined by image analysis of SEM photomicrographs. An enhanced phagocytic activity was observed after ozone exposure, with the greatest increase on Day 3. This enhanced phagocytic activity correlated with an increase in cell spreading. The results, which suggest that prolonged ozone insult produces an altered AM population, support previous morphological observations.

• Research Organization: Univ. of California, Davis
• OSTI ID: 6514069
• Journal Information: Environ. Res.; (United States), Vol. 28:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
MACROPHAGES
MORPHOLOGICAL CHANGES
OZONE
BIOLOGICAL EFFECTS
IN VITRO
LUNGS
PHAGOCYTOSIS
RATS
ANIMAL CELLS
ANIMALS
BODY
CONNECTIVE TISSUE CELLS
MAMMALS
ORGANS
PHAGOCYTES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)