Stabilization of enzymes for bioreactors. Final report on Phase 1
The objective of the Phase I project was to develop new stabilization techniques for immobilized enzymes used in bioreactors. The rationale was to provide an environment for optimal stability surrounding the enzyme, then to bind it together by copolymerization with vinyl monomers to obtain a large molecular weight, but soluble, enzyme derivative that would then be immobilized. The enzyme chosen for the project was beta-galactosidase from Aspergillus oryzae which is used in enzyme reactors for hydrolyzing lactose from milk whey. Several methods were used to achieve covalent crosslinking of beta-galactosidase. Although some stabilization was achieved with soluble, crosslinked beta-galactosidase, the methods originally proposed did not give significant stabilization of the immobilized enzyme. However, preliminary evidence was obtained that significant stabilization of immobilized beta-galactosidase was achieved by a modified crosslinking technique. The modified technique could have wide commercial applications for stabilizing enzymes used in bioreactors for the cheese and other dairy industries, food processing applications, pharmaceutical production, biodegradation of hazardous chemicals, and other scientific applications.
- Research Organization:
- Bio-Metric Systems, Inc., Eden Prairie, MN (United States)
- OSTI ID:
- 6502069
- Report Number(s):
- PB-93-173870/XAB; CNN: NSF-ISI-8861451
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BIOREACTORS
DESIGN
ENZYMES
STABILIZATION
ASPERGILLUS
BIODEGRADATION
CHROMATOGRAPHY
COMMERCIAL SECTOR
COPOLYMERIZATION
FOOD PROCESSING
GALACTOSIDASE
HYDROLYSIS
IMMOBILIZED ENZYMES
LACTOSE
RESEARCH PROGRAMS
VINYL MONOMERS
WHEY
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
EUMYCOTA
FOOD
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
INDUSTRIAL WASTES
LYSIS
MILK PRODUCTS
MONOMERS
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYMERIZATION
PROCESSING
PROTEINS
SACCHARIDES
SEPARATION PROCESSES
SOLVOLYSIS
WASTES
550200* - Biochemistry