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Title: Early events in herpes simplex virus type 1 infection: photosensitivity of fluorescein isothiocyanate-treated virions

Abstract

Herpes simplex virus type 1 is photosensitized by treatment with fluorescein isothiocyanate (FITC). The inactivation of FITC-treated virions upon subsequent exposure to light is inhibited by the presence of sodium azide, suggesting the involvement of singlet oxygen in the process. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed that treatment with FITC plus light induces crosslinks in viral envelope glycoproteins. Treatment of virions with high concentrations of FITC (50 ..mu..g/ml) plus light causes a reduction in the adsorption of the virus to monolayers of human embryonic lung cells. For lower concentrations of FITC (10 ..mu..g/ml) plus light, treated virions adsorb to the host cells, but remain sensitive to light until entry occurs. The loss of light sensitivity coincides with the development of resistance to antibodies. These results are most consistent with a mechanism of entry for herpes simplex virus involving fusion of the viral membrane with the plasma membrane of the host cell.

Authors:
; ; ;
Publication Date:
Research Org.:
Pennsylvania State Univ., University Park, PA (United States)
OSTI Identifier:
6501521
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 78:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; VIRUSES; INFECTIVITY; PHOTOSENSITIVITY; ADSORPTION; ANIMAL CELLS; ANTIBODIES; AZIDES; CELL CULTURES; CELL MEMBRANES; CROSS-LINKING; DETERGENTS; ELECTROPHORESIS; EXPERIMENTAL DATA; FLUORESCEIN; GLUCOPROTEINS; HERPES SIMPLEX; INACTIVATION; PHOTOREACTIVATION; SURVIVAL CURVES; THIOCYANATES; ADDITIVES; ANTITHYROID DRUGS; AROMATICS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; CARBOHYDRATES; CARBONIC ACID DERIVATIVES; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CHEMICAL REACTIONS; DATA; DISEASES; DRUGS; DYES; EMULSIFIERS; HORMONE ANTAGONISTS; HYDROXY ACIDS; HYDROXY COMPOUNDS; INFECTIOUS DISEASES; INFORMATION; MEMBRANES; MICROORGANISMS; NITROGEN COMPOUNDS; NUMERICAL DATA; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PARASITES; PHENOLS; POLYMERIZATION; POLYPHENOLS; PROTEINS; RECOVERY; REPAIR; SACCHARIDES; SENSITIVITY; SKIN DISEASES; SORPTION; SURFACTANTS; VIRAL DISEASES; WETTING AGENTS; 550700* - Microbiology; 550200 - Biochemistry

Citation Formats

DeLuca, N, Bzik, D, Person, S, and Snipes, W. Early events in herpes simplex virus type 1 infection: photosensitivity of fluorescein isothiocyanate-treated virions. United States: N. p., 1981. Web. doi:10.1073/pnas.78.2.912.
DeLuca, N, Bzik, D, Person, S, & Snipes, W. Early events in herpes simplex virus type 1 infection: photosensitivity of fluorescein isothiocyanate-treated virions. United States. https://doi.org/10.1073/pnas.78.2.912
DeLuca, N, Bzik, D, Person, S, and Snipes, W. 1981. "Early events in herpes simplex virus type 1 infection: photosensitivity of fluorescein isothiocyanate-treated virions". United States. https://doi.org/10.1073/pnas.78.2.912.
@article{osti_6501521,
title = {Early events in herpes simplex virus type 1 infection: photosensitivity of fluorescein isothiocyanate-treated virions},
author = {DeLuca, N and Bzik, D and Person, S and Snipes, W},
abstractNote = {Herpes simplex virus type 1 is photosensitized by treatment with fluorescein isothiocyanate (FITC). The inactivation of FITC-treated virions upon subsequent exposure to light is inhibited by the presence of sodium azide, suggesting the involvement of singlet oxygen in the process. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed that treatment with FITC plus light induces crosslinks in viral envelope glycoproteins. Treatment of virions with high concentrations of FITC (50 ..mu..g/ml) plus light causes a reduction in the adsorption of the virus to monolayers of human embryonic lung cells. For lower concentrations of FITC (10 ..mu..g/ml) plus light, treated virions adsorb to the host cells, but remain sensitive to light until entry occurs. The loss of light sensitivity coincides with the development of resistance to antibodies. These results are most consistent with a mechanism of entry for herpes simplex virus involving fusion of the viral membrane with the plasma membrane of the host cell.},
doi = {10.1073/pnas.78.2.912},
url = {https://www.osti.gov/biblio/6501521}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 78:2,
place = {United States},
year = {Sun Feb 01 00:00:00 EST 1981},
month = {Sun Feb 01 00:00:00 EST 1981}
}