Effects of the gamma-aminobutyrate transaminase inhibitors gabaculine and gamma-vinyl GABA on gamma-aminobutyric acid release from slices of rat cerebral cortex

Journal Article · Thu Jan 01 00:00:00 EST 1987 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00971363· OSTI ID:6501253

Bedwani, J R; Mehta, A

The release of (/sup 3/H)gamma-aminobutyric acid (GABA) from pre-loaded slices of rat cerebral cortex was investigated in the presence and absence of the GABA-transaminase inhibitors gabaculine and gamma-vinyl GABA. In the experiments carried out without an inhibitor, an ion-exchange column chromatographic technique was used to separate (/sup 3/H)GABA from tritiated metabolites released with it into the superfusate. The presence of gabaculine (5 microM) substantially reduced the Ca2+-dependence of the release of (/sup 3/H)GABA evoked by a 4 min 30 mM K+ pulse, whereas this was not appreciably reduced by the presence of gamma-vinyl GABA (2 mM or 10 mM). Nevertheless, the characteristics of (/sup 3/H)GABA release were not identical in the presence and absence of either inhibitor.

• Research Organization: Univ. College, Cardiff, Wales
• OSTI ID: 6501253
• Journal Information: Neurochem. Res.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINOBUTYRIC ACID
SECRETION
ENZYME INHIBITORS
BIOLOGICAL FUNCTIONS
AMINOTRANSFERASES
CALCIUM
CEREBRAL CORTEX
COMPARATIVE EVALUATIONS
ION EXCHANGE CHROMATOGRAPHY
METABOLITES
POTASSIUM
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALI METALS
ALKALINE EARTH METALS
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
BRAIN
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
CEREBRUM
CHROMATOGRAPHY
DRUGS
ELEMENTS
ENZYMES
FUNCTIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METALS
NERVOUS SYSTEM
NEUROREGULATORS
NITROGEN TRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
SEPARATION PROCESSES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques