Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and /sup 3/H- or /sup 125/I-labeled ligand compared

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:6500356

Wolf, B A; Daft, M C; Koenig, J W; Flye, M W; Turk, J W; Scott, M G

We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with /sup 3/H- or /sup 125/I-labeled cyclosporine ligand. The /sup 3/H-RIA kit slightly underestimated cyclosporine concentrations (greater than 600 micrograms/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the /sup 125/I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The /sup 125/I-RIA standard curve was linear to 1000 micrograms of cyclosporine per liter. Inter- and intra-assay CVs for /sup 125/I-RIA measurements of cyclosporine were less than or equal to 7%. Evidently, the /sup 125/I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO (USA)
• OSTI ID: 6500356
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 35:1
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
IMMUNOSUPPRESSIVE DRUGS
MEASURING METHODS
MONOCLONAL ANTIBODIES
SPECIFICITY
BLOOD
COMPARATIVE EVALUATIONS
HEART
IODINE 125
LIGANDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
RADIOIMMUNOASSAY
REGRESSION ANALYSIS
TRITIUM COMPOUNDS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MATERIALS
MATHEMATICS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SEPARATION PROCESSES
STATISTICS
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques