Stimulation of prostaglandin E/sub 2/ production by phorbol esters and epidermal growth factor in porcine thyroid cells
Effects of phorbol esters and epidermal growth factor (EGF) on prostaglandin E/sub 2/ production by cultured porcine thyroid cells were examined. Both phorbol 12-myristate 13-acetate (PMA) and EGF stimulated prostaglandin E/sub 2/ production by the cells in dose related fashion. PMA stimulated prostaglandin E/sub 2/ production over fifty-fold with the dose of 10/sup -7/ M compared with control. EGF (10/sup -7/ M) also stimulated it about ten-fold. The ED/sub 50/ values of PMA and EGF were respectively around 1 x 10/sup -9/ M and 5 x 10/sup -10/ M. Thyroid stimulating hormone (TSH), however, did not stimulate prostaglandin E/sub 2/ production from 1 to 24-h incubation. The release of radioactivity from (/sup 3/H)-arachidonic acid prelabeled cells was also stimulated by PMA and EGF, but not by TSH. These results indicate that both PMA and EGF are potent stimulators of prostaglandin E/sub 2/ production, associated with the activity to stimulate arachidonic acid release in porcine thyroid cells. 36 references, 2 figures, 1 table.
- Research Organization:
- Dokkyo Univ. School of Medicine, Tochigi, Japan
- OSTI ID:
- 6497039
- Journal Information:
- Life Sci.; (United States), Vol. 41:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PEPTIDE HORMONES
BIOLOGICAL EFFECTS
PHORBOL ESTERS
PROSTAGLANDINS
BIOSYNTHESIS
ARACHIDONIC ACID
DOSE-RESPONSE RELATIONSHIPS
EXPERIMENTAL DATA
SWINE
THYROID
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CARCINOGENS
DATA
DOMESTIC ANIMALS
ENDOCRINE GLANDS
ESTERS
GLANDS
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
SYNTHESIS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques