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Title: Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: an in vitro study

Abstract

The 577-nm flashlamp-pumped tunable dye laser pulsed at 450 microseconds is rapidly becoming the treatment of choice for removal of portwine stains and other vascular ectasias. In this study, we examined the mechanisms of vessel destruction by determining the effects of laser irradiation on three types of primary target cells--erythrocytes, endothelial cells, and fibroblasts. Human endothelial cells and fibroblasts in microwell plates were irradiated at various energy densities with the laser, after which several aspects of cellular biology were determined, including 1) viability of cells by trypan blue exclusion test; 2) cell proliferation by (3H)thymidine incorporation; and 3) rate of protein synthesis using (3H)leucine incorporation as a marker. In endothelial cell cultures, both (3H)thymidine and (3H)leucine incorporations were inhibited at energy levels of 5-12 J/cm2 (P less than 0.01). In fibroblast cultures, cell proliferation was similarly inhibited, while supratherapeutic energy density (greater than or equal to 12 J/cm2) was required for inhibition of protein synthesis. The laser energy in the range of 5-8.5 J/cm2 had no effect on cell viability. Erythrocytes as target cells for laser energy demonstrated rapid, dose-dependent lysis, as determined by release of free hemoglobin into culture medium. Addition of erythrocytes into a coculture with endothelial cellsmore » abolished the direct inhibitory effect noted in cultures when endothelial cells were present alone. The results of the latter experiment imply that erythrocytes are the primary target cell absorbing the laser energy at 577 nm. However, direct laser effects on endothelial cells may also contribute to the mechanisms of ablation of the vascular ectasias by the tunable dye laser at 577 nm.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Jefferson Medical College, Philadelphia, PA (USA)
OSTI Identifier:
6496102
Resource Type:
Journal Article
Journal Name:
Lasers Surg. Med.; (United States)
Additional Journal Information:
Journal Volume: 8:6
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; 62 RADIOLOGY AND NUCLEAR MEDICINE; ERYTHROCYTES; CELL PROLIFERATION; FIBROBLASTS; LASER RADIATION; BIOLOGICAL EFFECTS; DOSE-RESPONSE RELATIONSHIPS; DYE LASERS; ENDOTHELIUM; IN VITRO; LEUCINE; LYSIS; SURGERY; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; VIABILITY; AMINO ACIDS; ANIMAL CELLS; ANIMAL TISSUES; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LASERS; LIQUID LASERS; MATERIALS; MEDICINE; NUCLEOSIDES; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATIONS; RIBOSIDES; SOMATIC CELLS; TISSUES; 560400* - Other Environmental Pollutant Effects; 550901 - Pathology- Tracer Techniques; 550600 - Medicine

Citation Formats

Glassberg, E, Lask, G P, Tan, E M, and Uitto, J. Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: an in vitro study. United States: N. p., 1988. Web. doi:10.1002/lsm.1900080605.
Glassberg, E, Lask, G P, Tan, E M, & Uitto, J. Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: an in vitro study. United States. https://doi.org/10.1002/lsm.1900080605
Glassberg, E, Lask, G P, Tan, E M, and Uitto, J. 1988. "Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: an in vitro study". United States. https://doi.org/10.1002/lsm.1900080605.
@article{osti_6496102,
title = {Cellular effects of the pulsed tunable dye laser at 577 nanometers on human endothelial cells, fibroblasts, and erythrocytes: an in vitro study},
author = {Glassberg, E and Lask, G P and Tan, E M and Uitto, J},
abstractNote = {The 577-nm flashlamp-pumped tunable dye laser pulsed at 450 microseconds is rapidly becoming the treatment of choice for removal of portwine stains and other vascular ectasias. In this study, we examined the mechanisms of vessel destruction by determining the effects of laser irradiation on three types of primary target cells--erythrocytes, endothelial cells, and fibroblasts. Human endothelial cells and fibroblasts in microwell plates were irradiated at various energy densities with the laser, after which several aspects of cellular biology were determined, including 1) viability of cells by trypan blue exclusion test; 2) cell proliferation by (3H)thymidine incorporation; and 3) rate of protein synthesis using (3H)leucine incorporation as a marker. In endothelial cell cultures, both (3H)thymidine and (3H)leucine incorporations were inhibited at energy levels of 5-12 J/cm2 (P less than 0.01). In fibroblast cultures, cell proliferation was similarly inhibited, while supratherapeutic energy density (greater than or equal to 12 J/cm2) was required for inhibition of protein synthesis. The laser energy in the range of 5-8.5 J/cm2 had no effect on cell viability. Erythrocytes as target cells for laser energy demonstrated rapid, dose-dependent lysis, as determined by release of free hemoglobin into culture medium. Addition of erythrocytes into a coculture with endothelial cells abolished the direct inhibitory effect noted in cultures when endothelial cells were present alone. The results of the latter experiment imply that erythrocytes are the primary target cell absorbing the laser energy at 577 nm. However, direct laser effects on endothelial cells may also contribute to the mechanisms of ablation of the vascular ectasias by the tunable dye laser at 577 nm.},
doi = {10.1002/lsm.1900080605},
url = {https://www.osti.gov/biblio/6496102}, journal = {Lasers Surg. Med.; (United States)},
number = ,
volume = 8:6,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}