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Title: Continuous ethanol production from Jerusalem artichoke tubers. I. Use of free cells of Kluyveromyces marxianus

Journal Article · · Biotechnol. Bioeng.; (United States)

The continuous fermentation of Jerusalem artichoke juice to ethanol by free cells of Kluyveromyces marxianus UCD (FST) 55-82 has been studied in a continuous-stirred tank bioreactor at 35 degrees C and pH 4.6. A maximum ethanol yield of 90% of the theoretical was obtained at a dilution rate of 0.05/h. About 95% of the sugars were utilized at dilution rates lower than 0.15/h. Volumetric ethanol productivity and volumetric biomass productivity reached maximum values of 7 g EtOH/L/h and 0.6 g dry wt/L/h, respectively, at a dilution rate of 0.2h. The maintenance energy coefficient for Kluyveromyces marxianus culture was found to be 0.46 g sugar/g biomass/h. Oscillatory behavior was observed following a change in dilution rate from a previous steady state and from batch to continuous culture. Values of specific ethanol production rate and specific sugar uptake were found to increase almost linearly with the increase of the dilution rate. The maximum specific ethanol production rate and maximum specific sugar uptake rate were found to be 2.6 g ethanol/g cell/h and 7.9 sugars/g cell/h, respectively. Washout occurred at a dilution rate of 0.41/h. (Refs. 21).

Research Organization:
Dept of Chemical and Biochemical Engineering, Faculty of Engineering Science, The Univ of Western Ontario, London, Ontario, Canada N6A 5B9
OSTI ID:
6493337
Journal Information:
Biotechnol. Bioeng.; (United States), Vol. 24:7
Country of Publication:
United States
Language:
English