Phosphorylation of titan and nebulin in skeletal muscle
The in vitro and in vivo phosphorylation of skeletal muscle titin and nebulin are examined. It has been proposed that these proteins are the fundamental components of an elastic cytoskeletal lattice within the sarcomere. Determinations of endogenous phosphate in titin and nebulin purified from rabbit back muscle revealed phosphate contents of 3.10 +/- 0.26 mol phosphate/mol titin and 4.63 +/- 0.43 mol phosphate/mol nebulin. Incubation of rabbit back muscle homogenate in the presence of gamma-/sup 32/P ATP resulted in the labeling of both titin and nebulin; labeling was enhanced by the addition of cAMP-dependent protein kinase. Similar results were obtained from the incubation of chemically skinned rabbit psoas fibers in the presence of labeled ATP. A time dependent increase in phosphate incorporation was observed. Purification of titin and nebulin from Xenopus laevis frog gastrocnemius revealed endogenous phosphate contents of 6.15 +/- 0.12 mol phosphate/mol titin and 9.67 +/- 1.5 mol phosphate/mol nebulin. Titin and nebulin labeling after in vivo injection of Xenopus laevis frogs with /sup 32/P-orthophosphate was demonstrated.
- Research Organization:
- Yale Univ., New Haven, CT (USA)
- OSTI ID:
- 6478969
- Resource Relation:
- Other Information: Thesis (Ph. D)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MUSCLES
BIOCHEMISTRY
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
PROTEINS
PHOSPHORYLATION
ATP
IN VITRO
IN VIVO
PHOSPHORUS 32
RABBITS
TIME DEPENDENCE
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ENZYMES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques