Contribution of hydroxyl radical to radiosensitization: a study of DNA damage

Journal Article · Sat Sep 01 00:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576326· OSTI ID:6470938

Skov, K A

Using the radioprotector dimethylsulfoxide, DMSO, as a scavenger of hydroxyl radicals, the proportions of DNA damage caused by OH were determined in mammalian cells irradiated in hypoxia with or without the radiosensitizers misonidazole and TAN or in air. Yields of both single-strand breaks (SSB) and base/sugar damage (MLS for Micrococcus luteus sensitive sites) were measured for each situation. Most of the damage enhanced by the sensitizers was found to be OH dependent, for both MLS and SSB classes of damage. The sensitizer enhancement ratios in the presence of scavenger and the degree of protection afforded by the scavenger determined for total (MSL + SSB) damage agree well with those derived from corresponding survival experiments.

• Research Organization: British Columbia Cancer Research Center, Vancouver
• OSTI ID: 6470938
• Journal Information: Radiat. Res.; (United States), Vol. 99:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
DMSO
RADIOSENSITIVITY EFFECTS
HYDROXYL RADICALS
SCAVENGING
MISONIDAZOLE
STRAND BREAKS
RADIOINDUCTION
TAN
ANOXIA
CARBON 14 COMPOUNDS
CHO CELLS
DNA
EXPERIMENTAL DATA
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANTINEOPLASTIC DRUGS
AZINES
AZOLES
DATA
DRUGS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INFORMATION
KETONES
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PIPERIDINES
PYRIDINES
RADICALS
RADIOSENSITIZERS
SULFOXIDES
560121* - Radiation Effects on Cells- External Source- (-1987)
550300 - Cytology