cDNA sequences of two apolipoproteins from lamprey
The messages for two small but abundant apolipoproteins found in lamprey blood plasma were cloned with the aid of oligonucleotide probes based on amino-terminal sequences. In both cases, numerous clones were identified in a lamprey liver cDNA library, consistent with the great abundance of these proteins in lamprey blood. One of the cDNAs (LAL1) has a coding region of 105 amino acids that corresponds to a 21-residue signal peptide, a putative 8-residue propeptide, and the 76-residue mature protein found in blood. The other cDNA (LAL2) codes for a total of 191 residues, the first 23 of which constitute a signal peptide. The two proteins, which occur in the high-density lipoprotein fraction of ultracentrifuged plasma, have amino acid compositions similar to those of apolipoproteins found in mammalian blood; computer analysis indicates that the sequences are largely helix-permissive. When the sequences were searched against an amino acid sequence data base, rat apolipoprotein IV was the best matching candidate in both cases. Although a reasonable alignment can be made with that sequence and LAL1, definitive assignment of the two lamprey proteins to typical mammalian classes cannot be made at this point.
- Research Organization:
- Univ. of California, San Diego, La Jolla
- OSTI ID:
- 6470422
- Journal Information:
- Biochemistry; (United States), Vol. 26:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
APOLIPOPROTEINS
GENETICS
RECOMBINANT DNA
AUTORADIOGRAPHY
AMINO ACID SEQUENCE
ATP
BLOOD PLASMA
COMPUTER CALCULATIONS
DNA SEQUENCING
ELECTROPHORESIS
HYBRIDIZATION
LIVER
OLIGONUCLEOTIDES
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGY
BLOOD
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
GLANDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
LIPOPROTEINS
MATERIALS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques