cDNA sequences of two apolipoproteins from lamprey
Abstract
The messages for two small but abundant apolipoproteins found in lamprey blood plasma were cloned with the aid of oligonucleotide probes based on amino-terminal sequences. In both cases, numerous clones were identified in a lamprey liver cDNA library, consistent with the great abundance of these proteins in lamprey blood. One of the cDNAs (LAL1) has a coding region of 105 amino acids that corresponds to a 21-residue signal peptide, a putative 8-residue propeptide, and the 76-residue mature protein found in blood. The other cDNA (LAL2) codes for a total of 191 residues, the first 23 of which constitute a signal peptide. The two proteins, which occur in the high-density lipoprotein fraction of ultracentrifuged plasma, have amino acid compositions similar to those of apolipoproteins found in mammalian blood; computer analysis indicates that the sequences are largely helix-permissive. When the sequences were searched against an amino acid sequence data base, rat apolipoprotein IV was the best matching candidate in both cases. Although a reasonable alignment can be made with that sequence and LAL1, definitive assignment of the two lamprey proteins to typical mammalian classes cannot be made at this point.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of California, San Diego, La Jolla
- OSTI Identifier:
- 6470422
- Resource Type:
- Journal Article
- Journal Name:
- Biochemistry; (United States)
- Additional Journal Information:
- Journal Volume: 26:6
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; APOLIPOPROTEINS; GENETICS; RECOMBINANT DNA; AUTORADIOGRAPHY; AMINO ACID SEQUENCE; ATP; BLOOD PLASMA; COMPUTER CALCULATIONS; DNA SEQUENCING; ELECTROPHORESIS; HYBRIDIZATION; LIVER; OLIGONUCLEOTIDES; PHOSPHORUS 32; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BIOLOGY; BLOOD; BODY; BODY FLUIDS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; DNA; GLANDS; ISOTOPES; LIGHT NUCLEI; LIPIDS; LIPOPROTEINS; MATERIALS; MOLECULAR STRUCTURE; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; 550201* - Biochemistry- Tracer Techniques
Citation Formats
Pontes, M, Xu, X, Graham, D, Riley, M, and Doolittle, R F. cDNA sequences of two apolipoproteins from lamprey. United States: N. p., 1987.
Web. doi:10.1021/bi00380a019.
Pontes, M, Xu, X, Graham, D, Riley, M, & Doolittle, R F. cDNA sequences of two apolipoproteins from lamprey. United States. https://doi.org/10.1021/bi00380a019
Pontes, M, Xu, X, Graham, D, Riley, M, and Doolittle, R F. 1987.
"cDNA sequences of two apolipoproteins from lamprey". United States. https://doi.org/10.1021/bi00380a019.
@article{osti_6470422,
title = {cDNA sequences of two apolipoproteins from lamprey},
author = {Pontes, M and Xu, X and Graham, D and Riley, M and Doolittle, R F},
abstractNote = {The messages for two small but abundant apolipoproteins found in lamprey blood plasma were cloned with the aid of oligonucleotide probes based on amino-terminal sequences. In both cases, numerous clones were identified in a lamprey liver cDNA library, consistent with the great abundance of these proteins in lamprey blood. One of the cDNAs (LAL1) has a coding region of 105 amino acids that corresponds to a 21-residue signal peptide, a putative 8-residue propeptide, and the 76-residue mature protein found in blood. The other cDNA (LAL2) codes for a total of 191 residues, the first 23 of which constitute a signal peptide. The two proteins, which occur in the high-density lipoprotein fraction of ultracentrifuged plasma, have amino acid compositions similar to those of apolipoproteins found in mammalian blood; computer analysis indicates that the sequences are largely helix-permissive. When the sequences were searched against an amino acid sequence data base, rat apolipoprotein IV was the best matching candidate in both cases. Although a reasonable alignment can be made with that sequence and LAL1, definitive assignment of the two lamprey proteins to typical mammalian classes cannot be made at this point.},
doi = {10.1021/bi00380a019},
url = {https://www.osti.gov/biblio/6470422},
journal = {Biochemistry; (United States)},
number = ,
volume = 26:6,
place = {United States},
year = {Tue Mar 24 00:00:00 EST 1987},
month = {Tue Mar 24 00:00:00 EST 1987}
}