Stimulation of aortic smooth muscle cell mitogenesis by serotonin

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.3.674· OSTI ID:6470123

Nemecek, G M; Coughlin, S R; Handley, D A; Moskowitz, M A

Bovine aortic smooth muscle cells in vitro responded to 1 nM to 10 ..mu..M serotonin with increased incorporation of (/sup 3/H)thymidine into DNA. The mitogenic effect of serotonin was half-maximal at 80 nM and maximal above 1 ..mu..M. At a concentration of 1 ..mu..M, serotonin stimulated smooth muscle cell mitogenesis to the same extent as human platelet-derived growth factor (PDGF) at 12 ng/ml. Tryptamine was approx. = 1/10th as potent as serotonin as a mitogen for smooth muscle cells. Other indoles that are structurally related to serotonin (D- and L-tryptophan, 5-hydroxy-L-tryptophan, N-acetyl-5-hydroxytryptamine, melatonin, 5-hydroxyindoleacetic acid, and 5-hydroxytryptophol) and quipazine were inactive. The stimulatory effect of serotonin on smooth muscle cell DNA synthesis required prolonged (20-24 hr) exposure to the agonist and was attenuated in the presence of serotonin D receptor antagonists. When smooth muscle cells were incubated with submaximal concentrations of serotonin and PDGF, synergistic rather than additive mitogenic responses were observed. These data indicate that serotonin has a significant mitogenic effect on smooth muscle cells in vitro, which appears to be mediated by specific plasma membrane receptors.

• Research Organization: Sandoz Research Institute, East Hanover, NJ
• OSTI ID: 6470123
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:3
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
MUSCLES
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SEROTONIN
BIOLOGICAL EFFECTS
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CATTLE
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IN VITRO
MITOGENS
THYMIDINE
TRITIUM COMPOUNDS
TRYPTAMINES
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ANIMALS
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DOMESTIC ANIMALS
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INDOLES
LABELLED COMPOUNDS
MAMMALS
NEUROREGULATORS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
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PYRROLES
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550301* - Cytology- Tracer Techniques