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Title: Epidermal growth factor-nonresponsive 3T3 variants do not contain epidermal growth factor receptor-related antigens or mRNA

Abstract

The authors have previously isolated three independent variants of Swiss 3T3 cells that are unable to generate a mitogenic response to epidermal growth factor (EGF). Each of the variants is unable to bind /sup 125/I-labeled EGF; each lacks a functional EGF receptor. They used an antiserum to murine EGF receptor to look for an EGF-receptor gene product in wild-type 3T3 cells and in the three EGF-nonresponsive variants. No cross-reactive material could be detected in any of the three variants, either in /sup 125/I-labeled cell extracts or in (/sup 35/S)methionine metabolically labeled cells. 3T3 cells contained mRNA molecules homologous to a cDNA probe for the human EGF-receptor coding region. In contrast, no homologous RNA could be detected in any of the three variants. Analysis of genomic Southern blots of the DNA from 3T3 cells and the three EGF-nonresponsive variants indicated sequences from the EGF-receptor gene are present in the DNA of all four cell lines. These EGF-nonresponsive lines, which demonstrate proliferative responses to a variety of mitogens, will be ideal recipients for structure-function studies of the EGF receptor by transfection of the cloned gene.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of California, Los Angeles, CA (United States)
OSTI Identifier:
6469465
DOE Contract Number:  
AC03-76SF00012
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 83:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; FIBROBLASTS; IMMUNOLOGY; PROTEINS; RADIORECEPTOR ASSAY; ANIMAL GROWTH; ANTIGENS; DNA; IODINE 125; MESSENGER-RNA; METHIONINE; RECEPTORS; SULFUR 35; AMINO ACIDS; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; GROWTH; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MEMBRANE PROTEINS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; RADIOISOTOPES; RNA; SOMATIC CELLS; SULFUR ISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Schneider, C A, Lim, R W, Terwilliger, E, and Herschman, H R. Epidermal growth factor-nonresponsive 3T3 variants do not contain epidermal growth factor receptor-related antigens or mRNA. United States: N. p., 1986. Web. doi:10.1073/pnas.83.2.333.
Schneider, C A, Lim, R W, Terwilliger, E, & Herschman, H R. Epidermal growth factor-nonresponsive 3T3 variants do not contain epidermal growth factor receptor-related antigens or mRNA. United States. https://doi.org/10.1073/pnas.83.2.333
Schneider, C A, Lim, R W, Terwilliger, E, and Herschman, H R. 1986. "Epidermal growth factor-nonresponsive 3T3 variants do not contain epidermal growth factor receptor-related antigens or mRNA". United States. https://doi.org/10.1073/pnas.83.2.333.
@article{osti_6469465,
title = {Epidermal growth factor-nonresponsive 3T3 variants do not contain epidermal growth factor receptor-related antigens or mRNA},
author = {Schneider, C A and Lim, R W and Terwilliger, E and Herschman, H R},
abstractNote = {The authors have previously isolated three independent variants of Swiss 3T3 cells that are unable to generate a mitogenic response to epidermal growth factor (EGF). Each of the variants is unable to bind /sup 125/I-labeled EGF; each lacks a functional EGF receptor. They used an antiserum to murine EGF receptor to look for an EGF-receptor gene product in wild-type 3T3 cells and in the three EGF-nonresponsive variants. No cross-reactive material could be detected in any of the three variants, either in /sup 125/I-labeled cell extracts or in (/sup 35/S)methionine metabolically labeled cells. 3T3 cells contained mRNA molecules homologous to a cDNA probe for the human EGF-receptor coding region. In contrast, no homologous RNA could be detected in any of the three variants. Analysis of genomic Southern blots of the DNA from 3T3 cells and the three EGF-nonresponsive variants indicated sequences from the EGF-receptor gene are present in the DNA of all four cell lines. These EGF-nonresponsive lines, which demonstrate proliferative responses to a variety of mitogens, will be ideal recipients for structure-function studies of the EGF receptor by transfection of the cloned gene.},
doi = {10.1073/pnas.83.2.333},
url = {https://www.osti.gov/biblio/6469465}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 83:2,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}