Ethanologenic enzymes of Zymomonas mobilis: Progress report
In this study, we have proposed to investigate the regulatory mechanisms which permit the high level expression of the ethanologenic enzymes from Zymomonas mobilis (PDC, ADHI, ADHII). This research is continuing essentially as proposed in the original grant except that the scope is being expanded to include the glycolytic enzymes which are also highly expressed. Several enzymes which are expressed only at moderate levels are being examined for comparison (tryptophan biosynthesis, acid phosphatase). Studies of highly expressed genes involve enzyme purification and the production of antibodies, investigations of the effects of growth conditions on expression, cloning and characterization of structural genes, construction of hybrid genes, mutation of alcohol dehydrogenases, and investigation of transcriptional and translational regulation. In addition, we are investigating the feasibility of replacing the NAD regeneration systems of other bacteria with an artificial operon containing the Z. mobilis genes (PDC and ADHII) for the production of ethanol, the ''PET'' operon. 30 refs.
- Research Organization:
- Florida Univ., Gainesville (USA). Dept. of Microbiology and Cell Science
- DOE Contract Number:
- FG05-86ER13574
- OSTI ID:
- 6469239
- Report Number(s):
- DOE/ER/13574-3; ON: DE89008087
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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