Reproducible establishment of hemopoietic supportive stromal cell lines from murine bone marrow

Journal Article · Wed Feb 01 00:00:00 EST 1989 · Exp. Hematol.; (United States)

OSTI ID:6467017

Itoh, K; Tezuka, H; Sakoda, H; Konno, M; Nagata, K; Uchiyama, T; Uchino, H; Mori, K J

Stromal cell lines, designated MS-1, -2, -3, -4, -5, -6, and -7 were established by irradiating the adherent cells in long-term bone marrow cultures with 900-rad x-rays. Two of the cell lines, MS-1 and MS-5, have the capacity to support the growth of hemopoietic stem cells (spleen colony-forming cells and granulocyte-macrophage colony-forming cells) for greater than 2 months in vitro. These two cell lines were alkaline phosphatase-, peroxidase-, and factor VIII-negative and positive for periodic acid-Schiff and nonspecific esterase. Extracellular matrix proteins such as fibronectin, laminin, and collagen type I were produced by these two cell lines. Neither MS-1 cell- nor MS-5 cell-conditioned medium supported the growth of hemopoietic stem cells, and hemopoietic stem cells were found preferentially to be under and on MS-1 and MS-5 layers rather than in suspension. Close contact with the MS-1 cell layer or the MS-5 cell layer appears to be essential in maintaining hemopoiesis in vitro. Conditioned media from MS-1 cells and MS-5 cells stimulated granulocyte colony formation from murine bone marrow cells in semisolid culture.

• Research Organization: Niigata Univ. (Japan)
• OSTI ID: 6467017
• Journal Information: Exp. Hematol.; (United States), Vol. 17:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
STEM CELLS
GROWTH
ADHESION
ALKALINE PHOSPHATASE
COLLAGEN
COLONY FORMATION
ESTERASES
IRRADIATION
MICE
PEROXIDASES
PROTEINS
X RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYMES
HYDROLASES
IONIZING RADIATIONS
MAMMALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PHOSPHATASES
RADIATION EFFECTS
RADIATIONS
RODENTS
SCLEROPROTEINS
SOMATIC CELLS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture