Genetic dependence of recombination in recD mutants of Escherichia coli

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Genetics; (United States)

OSTI ID:6466494

Lovett, S T; Luisi-DeLuca, C; Kolodner, R D

RecBCD enzyme has multiple activities including helicase, exonuclease and endonuclease activities. Mutations in the genes recB or recC, encoding two subunits of the enzyme, reduce the frequency of many types of recombinational events. Mutations in recD, encoding the third subunit, do not reduce recombination even though most of the activities of the RecBCD enzyme are severely reduced. In this study, the genetic dependence of different types of recombination in recD mutants has been investigated. The effects of mutations in genes in the RecBCD pathway (recA and recC) as well as the genes specific for the RecF pathway (recF, recJ, recN, recO, recQ, ruv and lexA) were tested on conjugational, transductional and plasmid recombination, and on UV survival. recD mutants were hyper-recombinogenic for all the monitored recombination events, especially those involving plasmids, and all recombination events in recD strains required recA and recC. In addition, unlike recD+ strains, chromosomal recombination events and the repair of UV damage to DNA in recD strains were dependent on one RecF pathway gene, recJ. Only a subset of the tested recombination events were affected by ruv, recN, recQ, recO and lexA mutations.

• Research Organization: Dana-Farber Cancer Institute, Boston, MA (USA)
• OSTI ID: 6466494
• Journal Information: Genetics; (United States), Vol. 120:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA HELICASES
ENZYME ACTIVITY
DNA-ASE
ESCHERICHIA COLI
GENETIC RADIATION EFFECTS
RADIATION INDUCED MUTANTS
GENE RECOMBINATION
BIOLOGICAL PATHWAYS
DNA
ENDONUCLEASES
GENES
GENOTYPE
MUTATION FREQUENCY
MUTATIONS
PLASMIDS
ULTRAVIOLET RADIATION
BACTERIA
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BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GENETIC EFFECTS
HYDROLASES
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
RADIATION EFFECTS
RADIATIONS
560130* - Radiation Effects on Microorganisms