Isolation and screening of yeasts that ferment D-xylose directly to ethanol
Natural habitats of yeasts were examined for the presence of strains able to produce ethanol from D-xylose. Black knots, insect frass, and tree exudates were screened by enrichment in liquid D-xylose-yeast extract medium. These and each D-xylose-assimilating yeast in a collection from cactus fruits and Drosophila spp. were tested for alcohol production from this sugar. Among the 412 isolates examined, 36 produced more than 1 g of ethanol liter/sup -1/ from 20 g of D-xylose liter/sup -1/, all under aerated conditions. Closer examination of the strains indicated that their time courses of D-xylose fermentation followed different patterns. Some strains produced more biomass than ethanol, and among these, ethanol may or may not be assimilated rapidly after depletion of D-xylose. Others produced more ethanol than biomass, but all catabolized ethanol after carbohydrate exhaustion. Ethanol production appeared best at low pH values and under mild aeration. Possible correlations between the nutritional profiles of the yeasts and their ability to produce ethanol from D-xylose were explored by multivariate analysis. D-Xylose appeared slightly better utilized by yeasts which rate poorly in terms of fermentation. The fermentation of D-glucose had no bearing on D-xylose fermentation. No specific nutritional trait could discriminate well between better D-xylose fermentors and other yeasts.
- Research Organization:
- Univ. of Western Ontario, London
- OSTI ID:
- 6457633
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Vol. 50:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ETHANOL
BIOSYNTHESIS
XYLOSE
FERMENTATION
YEASTS
PRODUCTIVITY
COMPARATIVE EVALUATIONS
ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
FUNGI
HYDROXY COMPOUNDS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
PLANTS
SACCHARIDES
SYNTHESIS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)