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Title: Phosphorus-31 NMR magnetization transfer measurements of metabolic reaction rates in the rat heart and kidney in vivo

This dissertation is concerned with the measurement of the rates of ATP synthesis in the rat kidney and of the creatine kinase catalyzed reaction in the rat heart in situ. Chronically implanted detection coils, employing a balanced matching configuration of capacitors in the tuned circuit, were used to obtain /sup 31/P NMR spectra from heart, kidney, and liver in situ. Gated spectra of heart obtained at systole and diastole and the effects of fructose on kidney and liver were studied. The ability to observe other nuclei using implanted coils is illustrated with /sup 39/K NMR spectra from kidney and muscle. The theoretical considerations of applying magnetization transfer techniques to intact organs are discussed with emphasis on the problems associated with multiple exchange reactions and compartmentation of reactants. Experimental measurements of the ATP synthesis rate (13 ..mu..mol/min/gm tissue) were compared to whole kidney oxygen consumption and Na/sup +/ reabsorption rates to derive ATP/O (0.8 to 1.7) and Na/sup +//ATP (4 to 10) values. The problems associated with ATP synthesis rate measurements in kidney, e.g., the heterogeneity of the inorganic phosphate resonance, are discussed and experiments to overcome these problems proposed.
Authors:
Publication Date:
OSTI Identifier:
6451478
Report Number(s):
LBL-18376
ON: DE85001195
DOE Contract Number:
AC03-76SF00098
Resource Type:
Technical Report
Resource Relation:
Other Information: Portions are illegible in microfiche products. Thesis
Research Org:
Lawrence Berkeley Lab., CA (USA)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ATP; BIOSYNTHESIS; HEART; NUCLEAR MAGNETIC RESONANCE; KIDNEYS; PHOSPHOTRANSFERASES; METABOLISM; BIOCHEMICAL REACTION KINETICS; CREATINE; EXPERIMENTAL DATA; IN VIVO; PHOSPHORUS 31; POTASSIUM 39; RATS; ALKALI METAL ISOTOPES; AMINO ACIDS; ANIMALS; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; DATA; ENZYMES; INFORMATION; ISOTOPES; KINETICS; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; NUCLEI; NUCLEOTIDES; NUMERICAL DATA; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PHOSPHORUS-GROUP TRANSFERASES; POTASSIUM ISOTOPES; REACTION KINETICS; RESONANCE; RODENTS; STABLE ISOTOPES; SYNTHESIS; TRANSFERASES; VERTEBRATES 550500* -- Metabolism