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Title: Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene

Abstract

The biochemistry and genetics of the naphthalene degradation pathway contained on plasmid NAH7 have been well characterized. However, not much is known about the substrate specificity of the enzymes of nah operons and whether the nah-encoded enzymes are capable of metabolizing higher polyaromatic hydrocarbons. This paper shows that NAH7 and NAH7-like plasmids can mediate metabolism of phenanthrene and anthracene as well as naphthalene. In addition, a mutant blocked in the nahG (salicylate hydroxylase) gene produced unidentified metabolites when it is grown in the presence of phenanthrene and anthracene. This implies that phenanthrene and anthracene are degraded through the nah plasmid-encoded system. 29 refs., 3 figs., 2 tabs.

Authors:
 [1]; ; ;  [2]
  1. Univ. of Tennessee, Knoxville (United States) IT Corp., Knoxville, TN (United States)
  2. Univ. of Tennessee, Knoxville (United States)
Publication Date:
OSTI Identifier:
6444971
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 59:6; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BIODEGRADATION; BIOLOGICAL PATHWAYS; PLASMIDS; POLYCYCLIC AROMATIC HYDROCARBONS; ANTHRACENE; ENZYMES; METABOLISM; MINERALIZATION; NAPHTHALENE; PHENANTHRENE; PSEUDOMONAS; AROMATICS; BACTERIA; CELL CONSTITUENTS; CHEMICAL REACTIONS; CONDENSED AROMATICS; DECOMPOSITION; HYDROCARBONS; MICROORGANISMS; ORGANIC COMPOUNDS; PROTEINS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Sanseverino, J, Applegate, B M, King, J M.H., and Sayler, G S. Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene. United States: N. p., 1993. Web.
Sanseverino, J, Applegate, B M, King, J M.H., & Sayler, G S. Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene. United States.
Sanseverino, J, Applegate, B M, King, J M.H., and Sayler, G S. 1993. "Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene". United States.
@article{osti_6444971,
title = {Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene},
author = {Sanseverino, J and Applegate, B M and King, J M.H. and Sayler, G S},
abstractNote = {The biochemistry and genetics of the naphthalene degradation pathway contained on plasmid NAH7 have been well characterized. However, not much is known about the substrate specificity of the enzymes of nah operons and whether the nah-encoded enzymes are capable of metabolizing higher polyaromatic hydrocarbons. This paper shows that NAH7 and NAH7-like plasmids can mediate metabolism of phenanthrene and anthracene as well as naphthalene. In addition, a mutant blocked in the nahG (salicylate hydroxylase) gene produced unidentified metabolites when it is grown in the presence of phenanthrene and anthracene. This implies that phenanthrene and anthracene are degraded through the nah plasmid-encoded system. 29 refs., 3 figs., 2 tabs.},
doi = {},
url = {https://www.osti.gov/biblio/6444971}, journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 59:6,
place = {United States},
year = {Tue Jun 01 00:00:00 EDT 1993},
month = {Tue Jun 01 00:00:00 EDT 1993}
}