YAC-assisted cloning of a putative G-protein mapping to the MHC class I region

Journal Article · Tue Dec 01 00:00:00 EST 1992 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80105-3· OSTI ID:6443571

Denizot, F ^[1]; Mattei, M G ^[2]; Vernet, C; Pontarotti, P; Chimini, G ^[3]

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1. INSERM-CNRS de Marseille-Luminy (France) CBM-CNRS, Marseille (France)
2. Hospital d'Enfants de la Timone, Marseille (France)
3. Centre d'Immunologie, Marseille (France)

The authors report the successful use of whole yeast artificial chromosomes (YACs) as probes for direct positional cloning of novel expressed sequences in a given genomic fragment. The class I region of the human major histocompatibility complex, in particular the chromosomal fragment spanning the HLA-E locus, was investigated. The screening of a cDNA library with a 210- kb-long YAC clone led to the identification of a new gene, positionally conserved in the major histocompatibility complex of the mouse genome and encoding a putative GTP binding protein. Although its precise function remains unknown, the interspecies conservation of both sequence and map position suggests a regulatory or functional link with the histocompatibility cluster. 27 refs., 6 figs.

• OSTI ID: 6443571
• Journal Information: Genomics; (United States), Vol. 14:4; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DETECTION
HISTOCOMPATIBILITY COMPLEX
BIOLOGICAL EVOLUTION
DNA HYBRIDIZATION
DNA-CLONING
ANTIGENS
BIOTECHNOLOGY
CLONING
GENETIC ENGINEERING
HYBRIDIZATION
NUCLEIC ACID HYBRIDIZATION
550400* - Genetics